Overview
| Uniprot ID | O75925 |
|---|
| Protein Name | E3 SUMO-protein ligase PIAS1 |
|---|
| Gene Name | PIAS1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 238 |
PGYLPPTKNGVEPKR |
Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor (PubMed:11583632, PubMed:11867732, PubMed:14500712, PubMed:15280358, PubMed:21965678, PubMed:36050397). Catalyzes sumoylation of various proteins, such as CEBPB, MRE11, MTA1, PTK2, PML and ZNF76 (PubMed:11583632, PubMed:11867732, PubMed:14500712, PubMed:15280358, PubMed:21965678, PubMed:36050397). Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway (PubMed:11583632, PubMed:11867732). In vitro, binds A/T-rich DNA (PubMed:15133049). The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context (PubMed:11583632, PubMed:11867732, PubMed:14500712, PubMed:21965678, PubMed:36050397). Mediates sumoylation of MRE11, stabilizing MRE11 on chromatin during end resection (PubMed:36050397). Sumoylates PML (at 'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation (By similarity). PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678). Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity). Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes (By similarity). Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumoylation activity (By similarity). Capable of binding to the core enhancer region TAAT box in the MYOD1 gene promoter (By similarity)
Protein Sequence
10
MADSAELKQM
20
VMSLRVSELQ
30
VLLGYAGRNK
40
HGRKHELLTK
50
ALHLLKAGCS
60
PAVQMKIKEL
70
YRRRFPQKIM
80
TPADLSIPNV
90
HSSPMPATLS
100
PSTIPQLTYD
110
GHPASSPLLP
120
VSLLGPKHEL
130
ELPHLTSALH
140
PVHPDIKLQK
150
LPFYDLLDEL
160
IKPTSLASDN
170
SQRFRETCFA
180
FALTPQQVQQ
190
ISSSMDISGT
200
KCDFTVQVQL
210
RFCLSETSCP
220
QEDHFPPNLC
230
VKVNTKPCSL
240
PGYLPPTKNG
250
VEPKRPSRPI
260
NITSLVRLST
270
TVPNTIVVSW
280
TAEIGRNYSM
290
AVYLVKQLSS
300
TVLLQRLRAK
310
GIRNPDHSRA
320
LIKEKLTADP
330
DSEIATTSLR
340
VSLLCPLGKM
350
RLTIPCRALT
360
CSHLQCFDAT
370
LYIQMNEKKP
380
TWVCPVCDKK
390
APYEHLIIDG
400
LFMEILKYCT
410
DCDEIQFKED
420
GTWAPMRSKK
430
EVQEVSASYN
440
GVDGCLSSTL
450
EHQVASHHQS
460
SNKNKKVEVI
470
DLTIDSSSDE
480
EEEEPSAKRT
490
CPSLSPTSPL
500
NNKGILSLPH
510
QASPVSRTPS
520
LPAVDTSYIN
530
TSLIQDYRHP
540
FHMTPMPYDL
550
QGLDFFPFLS
560
GDNQHYNTSL
570
LAAAAAAVSD
580
DQDLLHSSRF
590
FPYTSSQMFL
600
DQLSAGGSTS
610
LPTTNGSSSG
620
SNSSLVSSNS
630
LRESHSHTVT
640
NRSSTDTASI
650
FGIIPDIISL
D
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0005856 |
cytoskeleton |
| Cellular Component |
GO:0098978 |
glutamatergic synapse |
| Cellular Component |
GO:0034399 |
nuclear periphery |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0016605 |
PML body |
| Cellular Component |
GO:0099524 |
postsynaptic cytosol |
| Cellular Component |
GO:0099523 |
presynaptic cytosol |
| Molecular Function |
GO:0140297 |
DNA-binding transcription factor binding |
| Molecular Function |
GO:0019899 |
enzyme binding |
| Molecular Function |
GO:0019904 |
protein domain specific binding |
| Molecular Function |
GO:0061665 |
SUMO ligase activity |
| Molecular Function |
GO:0019789 |
SUMO transferase activity |
| Molecular Function |
GO:0000976 |
transcription cis-regulatory region binding |
| Molecular Function |
GO:0003712 |
transcription coregulator activity |
| Molecular Function |
GO:0003714 |
transcription corepressor activity |
| Molecular Function |
GO:0031625 |
ubiquitin protein ligase binding |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0007259 |
cell surface receptor signaling pathway via JAK-STAT |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0045444 |
fat cell differentiation |
| Biological Process |
GO:0000082 |
G1/S transition of mitotic cell cycle |
| Biological Process |
GO:0043066 |
negative regulation of apoptotic process |
| Biological Process |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0032436 |
positive regulation of proteasomal ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:1904377 |
positive regulation of protein localization to cell periphery |
| Biological Process |
GO:0033235 |
positive regulation of protein sumoylation |
| Biological Process |
GO:0051152 |
positive regulation of smooth muscle cell differentiation |
| Biological Process |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0060261 |
positive regulation of transcription initiation by RNA polymerase II |
| Biological Process |
GO:0016925 |
protein sumoylation |
| Biological Process |
GO:0065004 |
protein-DNA complex assembly |
| Biological Process |
GO:0042127 |
regulation of cell population proliferation |
| Biological Process |
GO:0006357 |
regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0007283 |
spermatogenesis |
| Biological Process |
GO:0008542 |
visual learning |
Reference
PMID: N/A