Search Results

Overview

Uniprot IDO88989
Protein NameMalate dehydrogenase, cytoplasmic
Gene NameMdh1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
103 MERKDLLKANVKIFK
110 KANVKIFKSQGAALE
118 SQGAALEKYAKKSVK
142 TNCLTASKSAPSIPK
149 KSAPSIPKENFSCLT
164 RLDHNRAKSQIALKL
170 AKSQIALKLGVTADD
199 YPDVNHAKVKLQGKE
205 AKVKLQGKEVGVYEA
236 QRGAAVIKARKLSSA
239 AAVIKARKLSSAMSA
298 VIKNKTWKFVEGLPI
312 INDFSREKMDLTAKE
318 EKMDLTAKELTEEKE

Function

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation. Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS)

Protein Sequence

10 MSEPIRVLVT 20 GAAGQIAYSL 30 LYSIGNGSVF 40 GKDQPIILVL 50 LDITPMMGVL 60 DGVLMELQDC 70 ALPLLQDVIA 80 TDKEEVAFKD 90 LDVAVLVGSM 100 PRREGMERKD 110 LLKANVKIFK 120 SQGAALEKYA 130 KKSVKVIVVG 140 NPANTNCLTA 150 SKSAPSIPKE 160 NFSCLTRLDH 170 NRAKSQIALK 180 LGVTADDVKN 190 VIIWGNHSST 200 QYPDVNHAKV 210 KLQGKEVGVY 220 EALKDDSWLK 230 GEFITTVQQR 240 GAAVIKARKL 250 SSAMSAAKAI 260 SDHIRDIWFG 270 TPEGEFVSMG 280 VISDGNSYGV 290 PDDLLYSFPV 300 VIKNKTWKFV 310 EGLPINDFSR 320 EKMDLTAKEL 330 TEEKETAFEF LSSA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Molecular Function GO:0047995 (2R)-hydroxyphenylpyruvate reductase [NAD(P)H] activity
Molecular Function GO:0030060 L-malate dehydrogenase (NAD+) activity
Molecular Function GO:0016615 malate dehydrogenase activity
Molecular Function GO:0051287 NAD binding
Biological Process GO:0006108 malate metabolic process
Biological Process GO:0043490 malate-aspartate shuttle
Biological Process GO:0019674 NAD+ metabolic process
Biological Process GO:0006739 NADP+ metabolic process
Biological Process GO:0006107 oxaloacetate metabolic process
Biological Process GO:0006099 tricarboxylic acid cycle

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[3] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.