Overview
| Uniprot ID | O94762 |
|---|
| Protein Name | ATP-dependent DNA helicase Q5 |
|---|
| Gene Name | RECQL5 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 743 |
SGGSSLAKGRASKKQ |
| 869 |
NPESQPQKRPRPSAK |
Function
DNA helicase that plays an important role in DNA replication, transcription and repair (PubMed:20643585, PubMed:22973052, PubMed:28100692). Probably unwinds DNA in a 3'-5' direction (Probable) (PubMed:28100692). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability (PubMed:20231364). Also associates with POLR1A and enforces the stability of ribosomal DNA arrays (PubMed:27502483). Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress (PubMed:22013166). Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81-EME1 endonuclease leading to mitotic DNA synthesis (PubMed:28575661). Required for efficient DNA repair, including repair of inter-strand cross-links (PubMed:23715498). Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination. A core helicase fragment (residues 11-609) binds preferentially to splayed duplex, looped and ssDNA (PubMed:28100692)
Protein Sequence
10
MSSHHTTFPF
20
DPERRVRSTL
30
KKVFGFDSFK
40
TPLQESATMA
50
VVKGNKDVFV
60
CMPTGAGKSL
70
CYQLPALLAK
80
GITIVVSPLI
90
ALIQDQVDHL
100
LTLKVRVSSL
110
NSKLSAQERK
120
ELLADLEREK
130
PQTKILYITP
140
EMAASSSFQP
150
TLNSLVSRHL
160
LSYLVVDEAH
170
CVSQWGHDFR
180
PDYLRLGALR
190
SRLGHAPCVA
200
LTATATPQVQ
210
EDVFAALHLK
220
KPVAIFKTPC
230
FRANLFYDVQ
240
FKELISDPYG
250
NLKDFCLKAL
260
GQEADKGLSG
270
CGIVYCRTRE
280
ACEQLAIELS
290
CRGVNAKAYH
300
AGLKASERTL
310
VQNDWMEEKV
320
PVIVATISFG
330
MGVDKANVRF
340
VAHWNIAKSM
350
AGYYQESGRA
360
GRDGKPSWCR
370
LYYSRNDRDQ
380
VSFLIRKEVA
390
KLQEKRGNKA
400
SDKATIMAFD
410
ALVTFCEELG
420
CRHAAIAKYF
430
GDALPACAKG
440
CDHCQNPTAV
450
RRRLEALERS
460
SSWSKTCIGP
470
SQGNGFDPEL
480
YEGGRKGYGD
490
FSRYDEGSGG
500
SGDEGRDEAH
510
KREWNLFYQK
520
QMQLRKGKDP
530
KIEEFVPPDE
540
NCPLKEASSR
550
RIPRLTVKAR
560
EHCLRLLEEA
570
LSSNRQSTRT
580
ADEADLRAKA
590
VELEHETFRN
600
AKVANLYKAS
610
VLKKVADIHR
620
ASKDGQPYDM
630
GGSAKSCSAQ
640
AEPPEPNEYD
650
IPPASHVYSL
660
KPKRVGAGFP
670
KGSCPFQTAT
680
ELMETTRIRE
690
QAPQPERGGE
700
HEPPSRPCGL
710
LDEDGSEPLP
720
GPRGEVPGGS
730
AHYGGPSPEK
740
KAKSSSGGSS
750
LAKGRASKKQ
760
QLLATAAHKD
770
SQSIARFFCR
780
RVESPALLAS
790
APEAEGACPS
800
CEGVQGPPMA
810
PEKYTGEEDG
820
AGGHSPAPPQ
830
TEECLRERPS
840
TCPPRDQGTP
850
EVQPTPAKDT
860
WKGKRPRSQQ
870
ENPESQPQKR
880
PRPSAKPSVV
890
AEVKGSVSAS
900
EQGTLNPTAQ
910
DPFQLSAPGV
920
SLKEAANVVV
930
KCLTPFYKEG
940
KFASKELFKG
950
FARHLSHLLT
960
QKTSPGRSVK
970
EEAQNLIRHF
980
FHGRARCESE
990
ADWHGLCGPQ
R
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005694 |
chromosome |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005657 |
replication fork |
| Cellular Component |
GO:0097550 |
transcription preinitiation complex |
| Molecular Function |
GO:0043138 |
3'-5' DNA helicase activity |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016887 |
ATP hydrolysis activity |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0003678 |
DNA helicase activity |
| Molecular Function |
GO:0009378 |
four-way junction helicase activity |
| Molecular Function |
GO:0004386 |
helicase activity |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0000993 |
RNA polymerase II complex binding |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0051304 |
chromosome separation |
| Biological Process |
GO:0006259 |
DNA metabolic process |
| Biological Process |
GO:0006281 |
DNA repair |
| Biological Process |
GO:0006260 |
DNA replication |
| Biological Process |
GO:0000724 |
double-strand break repair via homologous recombination |
| Biological Process |
GO:0000278 |
mitotic cell cycle |
| Biological Process |
GO:1990506 |
mitotic DNA-templated DNA replication |
| Biological Process |
GO:0034244 |
negative regulation of transcription elongation by RNA polymerase II |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.