Overview
| Uniprot ID | O94868 |
|---|
| Protein Name | F-BAR and double SH3 domains protein 2 |
|---|
| Gene Name | FCHSD2 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 24 |
IQVEQMTKLQAKHQA |
Function
Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor (PubMed:29887380). Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling (PubMed:30249660). Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits (PubMed:29887380). Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate (PubMed:29887380). When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex. Does not promote actin polymerization in the absence of membranes (PubMed:29887380)
Protein Sequence
10
MQPPPRKVKV
20
TQELKNIQVE
30
QMTKLQAKHQ
40
AECDLLEDMR
50
TFSQKKAAIE
60
REYAQGMQKL
70
ASQYLKRDWP
80
GVKADDRNDY
90
RSMYPVWKSF
100
LEGTMQVAQS
110
RMNICENYKN
120
FISEPARTVR
130
SLKEQQLKRC
140
VDQLTKIQTE
150
LQETVKDLAK
160
GKKKYFETEQ
170
MAHAVREKAD
180
IEAKSKLSLF
190
QSRISLQKAS
200
VKLKARRSEC
210
NSKATHARND
220
YLLTLAAANA
230
HQDRYYQTDL
240
VNIMKALDGN
250
VYDHLKDYLI
260
AFSRTELETC
270
QAVQNTFQFL
280
LENSSKVVRD
290
YNLQLFLQEN
300
AVFHKPQPFQ
310
FQPCDSDTSR
320
QLESETGTTE
330
EHSLNKEARK
340
WATRVAREHK
350
NIVHQQRVLN
360
DLECHGAAVS
370
EQSRAELEQK
380
IDEARENIRK
390
AEIIKLKAEA
400
RLDLLKQIGV
410
SVDTWLKSAM
420
NQVMEELENE
430
RWARPPAVTS
440
NGTLHSLNAD
450
TEREEGEEFE
460
DNMDVFDDSS
470
SSPSGTLRNY
480
PLTCKVVYSY
490
KASQPDELTI
500
EEHEVLEVIE
510
DGDMEDWVKA
520
RNKVGQVGYV
530
PEKYLQFPTS
540
NSLLSMLQSL
550
AALDSRSHTS
560
SNSTEAELVS
570
GSLNGDASVC
580
FVKALYDYEG
590
QTDDELSFPE
600
GAIIRILNKE
610
NQDDDGFWEG
620
EFNGRIGVFP
630
SVLVEELSAS
640
ENGDTPWMRE
650
IQISPSPKPH
660
ASLPPLPLYD
670
QPPSSPYPSP
680
DKRSSLYFPR
690
SPSANEKSLH
700
AESPGFSQAS
710
RHTPETSYGK
720
LRPVRAAPPP
730
PTQNHRRPAE
740
KIEDVEITLV
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0031594 |
neuromuscular junction |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0055037 |
recycling endosome |
| Cellular Component |
GO:0120043 |
stereocilium shaft |
| Molecular Function |
GO:0005547 |
phosphatidylinositol-3,4,5-trisphosphate binding |
| Molecular Function |
GO:0043325 |
phosphatidylinositol-3,4-bisphosphate binding |
| Biological Process |
GO:0072583 |
clathrin-dependent endocytosis |
| Biological Process |
GO:0061024 |
membrane organization |
| Biological Process |
GO:0007274 |
neuromuscular synaptic transmission |
| Biological Process |
GO:0030838 |
positive regulation of actin filament polymerization |
| Biological Process |
GO:2000601 |
positive regulation of Arp2/3 complex-mediated actin nucleation |
| Biological Process |
GO:0015031 |
protein transport |
| Biological Process |
GO:0030833 |
regulation of actin filament polymerization |
| Cellular Component |
GO:0070161 |
anchoring junction |
| Cellular Component |
GO:0005905 |
clathrin-coated pit |
Reference
[1] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.