Search Results

Overview

Uniprot IDO94906
Protein NamePre-mRNA-processing factor 6
Gene NamePRPF6
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
137 KYRMERPKIQQQFSD
146 QQQFSDLKRKLAEVT
178 QRNPRYEKLTPVPDS
61 RTVGDQMKKNQAADD
62 TVGDQMKKNQAADDD

Function

Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed:20118938, PubMed:21549338, PubMed:28781166). Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation

Protein Sequence

10 MNKKKKPFLG 20 MPAPLGYVPG 30 LGRGATGFTT 40 RSDIGPARDA 50 NDPVDDRHAP 60 PGKRTVGDQM 70 KKNQAADDDD 80 EDLNDTNYDE 90 FNGYAGSLFS 100 SGPYEKDDEE 110 ADAIYAALDK 120 RMDERRKERR 130 EQREKEEIEK 140 YRMERPKIQQ 150 QFSDLKRKLA 160 EVTEEEWLSI 170 PEVGDARNKR 180 QRNPRYEKLT 190 PVPDSFFAKH 200 LQTGENHTSV 210 DPRQTQFGGL 220 NTPYPGGLNT 230 PYPGGMTPGL 240 MTPGTGELDM 250 RKIGQARNTL 260 MDMRLSQVSD 270 SVSGQTVVDP 280 KGYLTDLNSM 290 IPTHGGDIND 300 IKKARLLLKS 310 VRETNPHHPP 320 AWIASARLEE 330 VTGKLQVARN 340 LIMKGTEMCP 350 KSEDVWLEAA 360 RLQPGDTAKA 370 VVAQAVRHLP 380 QSVRIYIRAA 390 ELETDIRAKK 400 RVLRKALEHV 410 PNSVRLWKAA 420 VELEEPEDAR 430 IMLSRAVECC 440 PTSVELWLAL 450 ARLETYENAR 460 KVLNKARENI 470 PTDRHIWITA 480 AKLEEANGNT 490 QMVEKIIDRA 500 ITSLRANGVE 510 INREQWIQDA 520 EECDRAGSVA 530 TCQAVMRAVI 540 GIGIEEEDRK 550 HTWMEDADSC 560 VAHNALECAR 570 AIYAYALQVF 580 PSKKSVWLRA 590 AYFEKNHGTR 600 ESLEALLQRA 610 VAHCPKAEVL 620 WLMGAKSKWL 630 AGDVPAARSI 640 LALAFQANPN 650 SEEIWLAAVK 660 LESENDEYER 670 ARRLLAKARS 680 SAPTARVFMK 690 SVKLEWVQDN 700 IRAAQDLCEE 710 ALRHYEDFPK 720 LWMMKGQIEE 730 QKEMMEKARE 740 AYNQGLKKCP 750 HSTPLWLLLS 760 RLEEKIGQLT 770 RARAILEKSR 780 LKNPKNPGLW 790 LESVRLEYRA 800 GLKNIANTLM 810 AKALQECPNS 820 GILWSEAIFL 830 EARPQRRTKS 840 VDALKKCEHD 850 PHVLLAVAKL 860 FWSQRKITKA 870 REWFHRTVKI 880 DSDLGDAWAF 890 FYKFELQHGT 900 EEQQEEVRKR 910 CESAEPRHGE 920 LWCAVSKDIA 930 NWQKKIGDIL 940 RLVAGRIKNT F

Gene Ontology

Classification GO ID Description
Cellular Component GO:0071013 catalytic step 2 spliceosome
Cellular Component GO:0005682 U5 snRNP
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0030674 protein-macromolecule adaptor activity
Molecular Function GO:0043021 ribonucleoprotein complex binding
Molecular Function GO:0003723 RNA binding
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0006403 RNA localization
Biological Process GO:0008380 RNA splicing
Biological Process GO:0000375 RNA splicing, via transesterification reactions
Biological Process GO:0000245 spliceosomal complex assembly
Biological Process GO:0000387 spliceosomal snRNP assembly
Biological Process GO:0000244 spliceosomal tri-snRNP complex assembly
Biological Process GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
Cellular Component GO:0016020 membrane
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005681 spliceosomal complex
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Cellular Component GO:0046540 U4/U6 x U5 tri-snRNP complex

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.