Search Results

Overview

Uniprot ID	O94916
Protein Name	Nuclear factor of activated T-cells 5
Gene Name	NFAT5
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
623	KRSSTIFKTTKSVGS

Function

Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes (PubMed:10377394, PubMed:11485737, PubMed:41652173). Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed:10377394, PubMed:11485737). Mediates the transcriptional response to hypertonicity (PubMed:10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed:22266867). Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA (PubMed:34049076)

Protein Sequence

10 MPSDFISLLS 20 ADLDLESPKS 30 LYSRESVYDL 40 LPKELQLPPS 50 RETSVASMSQ 60 TSGGEAGSPP 70 PAVVAADASS 80 APSSSSMGGA 90 CSSFTTSSSP 100 TIYSTSVTDS 110 KAMQVESCSS 120 AVGVSNRGVS 130 EKQLTSNTVQ 140 QHPSTPKRHT 150 VLYISPPPED 160 LLDNSRMSCQ 170 DEGCGLESEQ 180 SCSMWMEDSP 190 SNFSNMSTSS 200 YNDNTEVPRK 210 SRKRNPKQRP 220 GVKRRDCEES 230 NMDIFDADSA 240 KAPHYVLSQL 250 TTDNKGNSKA 260 GNGTLENQKG 270 TGVKKSPMLC 280 GQYPVKSEGK 290 ELKIVVQPET 300 QHRARYLTEG 310 SRGSVKDRTQ 320 QGFPTVKLEG 330 HNEPVVLQVF 340 VGNDSGRVKP 350 HGFYQACRVT 360 GRNTTPCKEV 370 DIEGTTVIEV 380 GLDPSNNMTL 390 AVDCVGILKL 400 RNADVEARIG 410 IAGSKKKSTR 420 ARLVFRVNIM 430 RKDGSTLTLQ 440 TPSSPILCTQ 450 PAGVPEILKK 460 SLHSCSVKGE 470 EEVFLIGKNF 480 LKGTKVIFQE 490 NVSDENSWKS 500 EAEIDMELFH 510 QNHLIVKVPP 520 YHDQHITLPV 530 SVGIYVVTNA 540 GRSHDVQPFT 550 YTPDPAAAGA 560 LNVNVKKEIS 570 SPARPCSFEE 580 AMKAMKTTGC 590 NLDKVNIIPN 600 ALMTPLIPSS 610 MIKSEDVTPM 620 EVTAEKRSST 630 IFKTTKSVGS 640 TQQTLENISN 650 IAGNGSFSSP 660 SSSHLPSENE 670 KQQQIQPKAY 680 NPETLTTIQT 690 QDISQPGTFP 700 AVSASSQLPN 710 SDALLQQATQ 720 FQTRETQSRE 730 ILQSDGTVVN 740 LSQLTEASQQ 750 QQQSPLQEQA 760 QTLQQQISSN 770 IFPSPNSVSQ 780 LQNTIQQLQA 790 GSFTGSTASG 800 SSGSVDLVQQ 810 VLEAQQQLSS 820 VLFSAPDGNE 830 NVQEQLSADI 840 FQQVSQIQSG 850 VSPGMFSSTE 860 PTVHTRPDNL 870 LPGRAESVHP 880 QSENTLSNQQ 890 QQQQQQQQVM 900 ESSAAMVMEM 910 QQSICQAAAQ 920 IQSELFPSTA 930 SANGNLQQSP 940 VYQQTSHMMS 950 ALSTNEDMQM 960 QCELFSSPPA 970 VSGNETSTTT 980 TQQVATPGTT 990 MFQTSSSGDG 1000 EETGTQAKQI 1010 QNSVFQTMVQ 1020 MQHSGDNQPQ 1030 VNLFSSTKSM 1040 MSVQNSGTQQ 1050 QGNGLFQQGN 1060 EMMSLQSGNF 1070 LQQSSHSQAQ 1080 LFHPQNPIAD 1090 AQNLSQETQG 1100 SLFHSPNPIV 1110 HSQTSTTSSE 1120 QMQPPMFHSQ 1130 STIAVLQGSS 1140 VPQDQQSTNI 1150 FLSQSPMNNL 1160 QTNTVAQEAF 1170 FAAPNSISPL 1180 QSTSNSEQQA 1190 AFQQQAPISH 1200 IQTPMLSQEQ 1210 AQPPQQGLFQ 1220 PQVALGSLPP 1230 NPMPQSQQGT 1240 MFQSQHSIVA 1250 MQSNSPSQEQ 1260 QQQQQQQQQQ 1270 QQQQQQSILF 1280 SNQNTMATMA 1290 SPKQPPPNMI 1300 FNPNQNPMAN 1310 QEQQNQSIFH 1320 QQSNMAPMNQ 1330 EQQPMQFQSQ 1340 STVSSLQNPG 1350 PTQSESSQTP 1360 LFHSSPQIQL 1370 VQGSPSSQEQ 1380 QVTLFLSPAS 1390 MSALQTSINQ 1400 QDMQQSPLYS 1410 PQNNMPGIQG 1420 ATSSPQPQAT 1430 LFHNTAGGTM 1440 NQLQNSPGSS 1450 QQTSGMFLFG 1460 IQNNCSQLLT 1470 SGPATLPDQL 1480 MAISQPGQPQ 1490 NEGQPPVTTL 1500 LSQQMPENSP 1510 LASSINTNQN 1520 IEKIDLLVSL 1530 QNQGNNLTGS F

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0000785	chromatin
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0005667	transcription regulator complex
Molecular Function	GO:0003682	chromatin binding
Molecular Function	GO:0001228	DNA-binding transcription activator activity, RNA polymerase II-specific
Molecular Function	GO:0003700	DNA-binding transcription factor activity
Molecular Function	GO:0000981	DNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function	GO:1990837	sequence-specific double-stranded DNA binding
Biological Process	GO:0033173	calcineurin-NFAT signaling cascade
Biological Process	GO:0071474	cellular hyperosmotic response
Biological Process	GO:0071345	cellular response to cytokine stimulus
Biological Process	GO:0006974	DNA damage response
Biological Process	GO:0043123	positive regulation of canonical NF-kappaB signal transduction
Biological Process	GO:1904996	positive regulation of leukocyte adhesion to vascular endothelial cell
Biological Process	GO:0045944	positive regulation of transcription by RNA polymerase II
Biological Process	GO:0062176	R-loop processing
Biological Process	GO:0007165	signal transduction
Biological Process	GO:0006366	transcription by RNA polymerase II

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.