Search Results

Overview

Uniprot IDO95243
Protein NameMethyl-CpG-binding domain protein 4
Gene NameMBD4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
239 KGKVTILKGIPIKKT
335 KTSGIINKFCSAKDS

Function

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein

Protein Sequence

10 MGTTGLESLS 20 LGDRGAAPTV 30 TSSERLVPDP 40 PNDLRKEDVA 50 MELERVGEDE 60 EQMMIKRSSE 70 CNPLLQEPIA 80 SAQFGATAGT 90 ECRKSVPCGW 100 ERVVKQRLFG 110 KTAGRFDVYF 120 ISPQGLKFRS 130 KSSLANYLHK 140 NGETSLKPED 150 FDFTVLSKRG 160 IKSRYKDCSM 170 AALTSHLQNQ 180 SNNSNWNLRT 190 RSKCKKDVFM 200 PPSSSSELQE 210 SRGLSNFTST 220 HLLLKEDEGV 230 DDVNFRKVRK 240 PKGKVTILKG 250 IPIKKTKKGC 260 RKSCSGFVQS 270 DSKRESVCNK 280 ADAESEPVAQ 290 KSQLDRTVCI 300 SDAGACGETL 310 SVTSEENSLV 320 KKKERSLSSG 330 SNFCSEQKTS 340 GIINKFCSAK 350 DSEHNEKYED 360 TFLESEEIGT 370 KVEVVERKEH 380 LHTDILKRGS 390 EMDNNCSPTR 400 KDFTGEKIFQ 410 EDTIPRTQIE 420 RRKTSLYFSS 430 KYNKEALSPP 440 RRKAFKKWTP 450 PRSPFNLVQE 460 TLFHDPWKLL 470 IATIFLNRTS 480 GKMAIPVLWK 490 FLEKYPSAEV 500 ARTADWRDVS 510 ELLKPLGLYD 520 LRAKTIVKFS 530 DEYLTKQWKY 540 PIELHGIGKY 550 GNDSYRIFCV 560 NEWKQVHPED 570 HKLNKYHDWL 580 WENHEKLSLS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0004520 DNA endonuclease activity
Molecular Function GO:0019104 DNA N-glycosylase activity
Molecular Function GO:0008263 pyrimidine-specific mismatch base pair DNA N-glycosylase activity
Molecular Function GO:0003696 satellite DNA binding
Biological Process GO:0045008 depyrimidination
Biological Process GO:0006281 DNA repair
Biological Process GO:0032355 response to estradiol

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.