Search Results

Overview

Uniprot IDO95251
Protein NameHistone acetyltransferase KAT7
Gene NameKAT7
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
263 RQLRYKEKVAELRKK
323 RASEDLEKLRLQGQI
90 QPTPVTPKKYPLRQT
91 PTPVTPKKYPLRQTR

Function

Catalytic subunit of histone acetyltransferase HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby regulating various processes, such as gene transcription, protein ubiquitination, immune regulation, stem cell pluripotent and self-renewal maintenance and embryonic development (PubMed:16387653, PubMed:21753189, PubMed:24065767, PubMed:26620551, PubMed:31767635, PubMed:31827282). Some complexes also catalyze acetylation of histone H4 at 'Lys-5', 'Lys-8' and 'Lys-12' (H4K5ac, H4K8ac and H4K12ac, respectively), regulating DNA replication initiation, regulating DNA replication initiation (PubMed:10438470, PubMed:19187766, PubMed:20129055, PubMed:24065767). Specificity of the HBO1 complexes is determined by the scaffold subunit: complexes containing BRPF scaffold (BRPF1, BRD1/BRPF2 or BRPF3) direct KAT7/HBO1 specificity towards H3K14ac, while complexes containing JADE (JADE1, JADE2 and JADE3) scaffold direct KAT7/HBO1 specificity towards histone H4 (PubMed:19187766, PubMed:20129055, PubMed:24065767, PubMed:26620551). H3K14ac promotes transcriptional elongation by facilitating the processivity of RNA polymerase II (PubMed:31827282). Acts as a key regulator of hematopoiesis by forming a complex with BRD1/BRPF2, directing KAT7/HBO1 specificity towards H3K14ac and promoting erythroid differentiation (PubMed:21753189). H3K14ac is also required for T-cell development (By similarity). KAT7/HBO1-mediated acetylation facilitates two consecutive steps, licensing and activation, in DNA replication initiation: H3K14ac facilitates the activation of replication origins, and histone H4 acetylation (H4K5ac, H4K8ac and H4K12ac) facilitates chromatin loading of MCM complexes, promoting DNA replication licensing (PubMed:10438470, PubMed:11278932, PubMed:18832067, PubMed:19187766, PubMed:20129055, PubMed:21856198, PubMed:24065767, PubMed:26620551). Acts as a positive regulator of centromeric CENPA assembly: recruited to centromeres and mediates histone acetylation, thereby preventing centromere inactivation mediated by SUV39H1, possibly by increasing histone turnover/exchange (PubMed:27270040). Involved in nucleotide excision repair: phosphorylation by ATR in response to ultraviolet irradiation promotes its localization to DNA damage sites, where it mediates histone acetylation to facilitate recruitment of XPC at the damaged DNA sites (PubMed:28719581). Acts as an inhibitor of NF-kappa-B independently of its histone acetyltransferase activity (PubMed:16997280)

Protein Sequence

10 MPRRKRNAGS 20 SSDGTEDSDF 30 STDLEHTDSS 40 ESDGTSRRSA 50 RVTRSSARLS 60 QSSQDSSPVR 70 NLQSFGTEEP 80 AYSTRRVTRS 90 QQQPTPVTPK 100 KYPLRQTRSS 110 GSETEQVVDF 120 SDRETKNTAD 130 HDESPPRTPT 140 GNAPSSESDI 150 DISSPNVSHD 160 ESIAKDMSLK 170 DSGSDLSHRP 180 KRRRFHESYN 190 FNMKCPTPGC 200 NSLGHLTGKH 210 ERHFSISGCP 220 LYHNLSADEC 230 KVRAQSRDKQ 240 IEERMLSHRQ 250 DDNNRHATRH 260 QAPTERQLRY 270 KEKVAELRKK 280 RNSGLSKEQK 290 EKYMEHRQTY 300 GNTREPLLEN 310 LTSEYDLDLF 320 RRAQARASED 330 LEKLRLQGQI 340 TEGSNMIKTI 350 AFGRYELDTW 360 YHSPYPEEYA 370 RLGRLYMCEF 380 CLKYMKSQTI 390 LRRHMAKCVW 400 KHPPGDEIYR 410 KGSISVFEVD 420 GKKNKIYCQN 430 LCLLAKLFLD 440 HKTLYYDVEP 450 FLFYVMTEAD 460 NTGCHLIGYF 470 SKEKNSFLNY 480 NVSCILTMPQ 490 YMRQGYGKML 500 IDFSYLLSKV 510 EEKVGSPERP 520 LSDLGLISYR 530 SYWKEVLLRY 540 LHNFQGKEIS 550 IKEISQETAV 560 NPVDIVSTLQ 570 ALQMLKYWKG 580 KHLVLKRQDL 590 IDEWIAKEAK 600 RSNSNKTMDP 610 SCLKWTPPKG T

Gene Ontology

Classification GO ID Description
Molecular Function GO:0008270 zinc ion binding
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005694 chromosome
Cellular Component GO:0000775 chromosome, centromeric region
Cellular Component GO:0005829 cytosol
Cellular Component GO:0000123 histone acetyltransferase complex
Cellular Component GO:0036409 histone H3-K14 acetyltransferase complex
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0090734 site of DNA damage
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0003688 DNA replication origin binding
Molecular Function GO:0004402 histone acetyltransferase activity
Molecular Function GO:0010484 histone H3 acetyltransferase activity
Molecular Function GO:0036408 histone H3K14 acetyltransferase activity
Molecular Function GO:0043994 histone H3K23 acetyltransferase activity
Molecular Function GO:0044016 histone H3K4 acetyltransferase activity
Molecular Function GO:0010485 histone H4 acetyltransferase activity
Molecular Function GO:0043997 histone H4K12 acetyltransferase activity
Molecular Function GO:0043995 histone H4K5 acetyltransferase activity
Molecular Function GO:0043996 histone H4K8 acetyltransferase activity
Molecular Function GO:0003712 transcription coregulator activity
Biological Process GO:0006281 DNA repair
Biological Process GO:0006260 DNA replication
Biological Process GO:0140889 DNA replication-dependent chromatin disassembly
Biological Process GO:0018393 internal peptidyl-lysine acetylation
Biological Process GO:0001779 natural killer cell differentiation
Biological Process GO:0045740 positive regulation of DNA replication
Biological Process GO:0032786 positive regulation of DNA-templated transcription, elongation
Biological Process GO:0045648 positive regulation of erythrocyte differentiation
Biological Process GO:1902035 positive regulation of hematopoietic stem cell proliferation
Biological Process GO:1900182 positive regulation of protein localization to nucleus
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0051726 regulation of cell cycle
Biological Process GO:0001558 regulation of cell growth
Biological Process GO:2000278 regulation of DNA biosynthetic process
Biological Process GO:0006275 regulation of DNA replication
Biological Process GO:0030174 regulation of DNA-templated DNA replication initiation
Biological Process GO:0006355 regulation of DNA-templated transcription
Biological Process GO:2000819 regulation of nucleotide-excision repair
Biological Process GO:0006357 regulation of transcription by RNA polymerase II
Biological Process GO:0072716 response to actinomycin D
Biological Process GO:0072739 response to anisomycin
Biological Process GO:0072720 response to dithiothreitol
Biological Process GO:0072710 response to hydroxyurea
Biological Process GO:0072708 response to sorbitol
Biological Process GO:0031098 stress-activated protein kinase signaling cascade
Biological Process GO:0030217 T cell differentiation
Biological Process GO:0045815 transcription initiation-coupled chromatin remodeling

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.