Overview
| Uniprot ID | O95340 |
|---|
| Protein Name | Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 |
|---|
| Gene Name | PAPSS2 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 8 |
MSGIKKQKTENQQKS |
Function
Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate/PAPS, the activated sulfate donor used by sulfotransferases (PubMed:11773860, PubMed:19474428, PubMed:23824674, PubMed:25594860). In mammals, PAPS is the sole source of sulfate while APS appears to only be an intermediate in the sulfate-activation pathway (PubMed:11773860, PubMed:19474428, PubMed:23824674, PubMed:25594860). Plays indirectly an important role in skeletogenesis during postnatal growth (PubMed:9771708)
Protein Sequence
10
MSGIKKQKTE
20
NQQKSTNVVY
30
QAHHVSRNKR
40
GQVVGTRGGF
50
RGCTVWLTGL
60
SGAGKTTISF
70
ALEEYLVSHA
80
IPCYSLDGDN
90
VRHGLNRNLG
100
FSPGDREENI
110
RRIAEVAKLF
120
ADAGLVCITS
130
FISPFAKDRE
140
NARKIHESAG
150
LPFFEIFVDA
160
PLNICESRDV
170
KGLYKRARAG
180
EIKGFTGIDS
190
DYEKPETPER
200
VLKTNLSTVS
210
DCVHQVVELL
220
QEQNIVPYTI
230
IKDIHELFVP
240
ENKLDHVRAE
250
AETLPSLSIT
260
KLDLQWVQVL
270
SEGWATPLKG
280
FMREKEYLQV
290
MHFDTLLDDG
300
VINMSIPIVL
310
PVSAEDKTRL
320
EGCSKFVLAH
330
GGRRVAILRD
340
AEFYEHRKEE
350
RCSRVWGTTC
360
TKHPHIKMVM
370
ESGDWLVGGD
380
LQVLEKIRWN
390
DGLDQYRLTP
400
LELKQKCKEM
410
NADAVFAFQL
420
RNPVHNGHAL
430
LMQDTRRRLL
440
ERGYKHPVLL
450
LHPLGGWTKD
460
DDVPLDWRMK
470
QHAAVLEEGV
480
LDPKSTIVAI
490
FPSPMLYAGP
500
TEVQWHCRSR
510
MIAGANFYIV
520
GRDPAGMPHP
530
ETKKDLYEPT
540
HGGKVLSMAP
550
GLTSVEIIPF
560
RVAAYNKAKK
570
AMDFYDPARH
580
NEFDFISGTR
590
MRKLAREGEN
600
PPDGFMAPKA
610
WKVLTDYYRS
LEKN
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0004020 |
adenylylsulfate kinase activity |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016779 |
nucleotidyltransferase activity |
| Molecular Function |
GO:0004781 |
sulfate adenylyltransferase (ATP) activity |
| Biological Process |
GO:0050428 |
3'-phosphoadenosine 5'-phosphosulfate biosynthetic process |
| Biological Process |
GO:0042445 |
hormone metabolic process |
| Biological Process |
GO:0000103 |
sulfate assimilation |
Reference
[1] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.