Search Results

Overview

Uniprot ID	O95671
Protein Name	Probable bifunctional dTTP/UTP pyrophosphatase/methyltransferase protein
Gene Name	ASMTL
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
225	EDLRRSVKHDSIPAA
409	HHRALGKKAEDLFQD
65	AMETAKQKALEVANR

Function

Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. Can also hydrolyze CTP and the modified nucleotides pseudo-UTP, 5-methyl-UTP (m(5)UTP) and 5-methyl-CTP (m(5)CTP). Has weak activity with dCTP, 8-oxo-GTP and N(4)-methyl-dCTP (PubMed:24210219). May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids (PubMed:24210219). In addition, the presence of the putative catalytic domain of S-adenosyl-L-methionine binding in the C-terminal region argues for a methyltransferase activity (Probable)

Protein Sequence

10 MVLCPVIGKL 20 LHKRVVLASA 30 SPRRQEILSN 40 AGLRFEVVPS 50 KFKEKLDKAS 60 FATPYGYAME 70 TAKQKALEVA 80 NRLYQKDLRA 90 PDVVIGADTI 100 VTVGGLILEK 110 PVDKQDAYRM 120 LSRLSGREHS 130 VFTGVAIVHC 140 SSKDHQLDTR 150 VSEFYEETKV 160 KFSELSEELL 170 WEYVHSGEPM 180 DKAGGYGIQA 190 LGGMLVESVH 200 GDFLNVVGFP 210 LNHFCKQLVK 220 LYYPPRPEDL 230 RRSVKHDSIP 240 AADTFEDLSD 250 VEGGGSEPTQ 260 RDAGSRDEKA 270 EAGEAGQATA 280 EAECHRTRET 290 LPPFPTRLLE 300 LIEGFMLSKG 310 LLTACKLKVF 320 DLLKDEAPQK 330 AADIASKVDA 340 SACGMERLLD 350 ICAAMGLLEK 360 TEQGYSNTET 370 ANVYLASDGE 380 YSLHGFIMHN 390 NDLTWNLFTY 400 LEFAIREGTN 410 QHHRALGKKA 420 EDLFQDAYYQ 430 SPETRLRFMR 440 AMHGMTKLTA 450 CQVATAFNLS 460 RFSSACDVGG 470 CTGALARELA 480 REYPRMQVTV 490 FDLPDIIELA 500 AHFQPPGPQA 510 VQIHFAAGDF 520 FRDPLPSAEL 530 YVLCRILHDW 540 PDDKVHKLLS 550 RVAESCKPGA 560 GLLLVETLLD 570 EEKRVAQRAL 580 MQSLNMLVQT 590 EGKERSLGEY 600 QCLLELHGFH 610 QVQVVHLGGV 620 LDAILATKVA P

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Molecular Function	GO:0036218	dTTP diphosphatase activity
Molecular Function	GO:0047429	nucleoside triphosphate diphosphatase activity
Molecular Function	GO:0008171	O-methyltransferase activity
Molecular Function	GO:0046983	protein dimerization activity
Molecular Function	GO:0036221	UTP diphosphatase activity
Biological Process	GO:0032259	methylation
Biological Process	GO:0009117	nucleotide metabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.