Overview
| Uniprot ID | O95819 |
| Protein Name | Mitogen-activated protein kinase kinase kinase kinase 4 |
| Gene Name | MAP4K4 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 670 |
LLWERVEKLVPRPGS |
Function
Serine/threonine kinase that plays a role in the response to environmental stress and cytokines such as TNF. Appears to act upstream of the JUN N-terminal pathway (PubMed:9890973). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed:26437443). Phosphorylates SMAD1 on Thr-322 (PubMed:21690388)
Protein Sequence
10
MANDSPAKSL
20
VDIDLSSLRD
30
PAGIFELVEV
40
VGNGTYGQVY
50
KGRHVKTGQL
60
AAIKVMDVTE
70
DEEEEIKLEI
80
NMLKKYSHHR
90
NIATYYGAFI
100
KKSPPGHDDQ
110
LWLVMEFCGA
120
GSITDLVKNT
130
KGNTLKEDWI
140
AYISREILRG
150
LAHLHIHHVI
160
HRDIKGQNVL
170
LTENAEVKLV
180
DFGVSAQLDR
190
TVGRRNTFIG
200
TPYWMAPEVI
210
ACDENPDATY
220
DYRSDLWSCG
230
ITAIEMAEGA
240
PPLCDMHPMR
250
ALFLIPRNPP
260
PRLKSKKWSK
270
KFFSFIEGCL
280
VKNYMQRPST
290
EQLLKHPFIR
300
DQPNERQVRI
310
QLKDHIDRTR
320
KKRGEKDETE
330
YEYSGSEEEE
340
EEVPEQEGEP
350
SSIVNVPGES
360
TLRRDFLRLQ
370
QENKERSEAL
380
RRQQLLQEQQ
390
LREQEEYKRQ
400
LLAERQKRIE
410
QQKEQRRRLE
420
EQQRREREAR
430
RQQEREQRRR
440
EQEEKRRLEE
450
LERRRKEEEE
460
RRRAEEEKRR
470
VEREQEYIRR
480
QLEEEQRHLE
490
VLQQQLLQEQ
500
AMLLECRWRE
510
MEEHRQAERL
520
QRQLQQEQAY
530
LLSLQHDHRR
540
PHPQHSQQPP
550
PPQQERSKPS
560
FHAPEPKAHY
570
EPADRAREVE
580
DRFRKTNHSS
590
PEAQSKQTGR
600
VLEPPVPSRS
610
ESFSNGNSES
620
VHPALQRPAE
630
PQVPVRTTSR
640
SPVLSRRDSP
650
LQGSGQQNSQ
660
AGQRNSTSIE
670
PRLLWERVEK
680
LVPRPGSGSS
690
SGSSNSGSQP
700
GSHPGSQSGS
710
GERFRVRSSS
720
KSEGSPSQRL
730
ENAVKKPEDK
740
KEVFRPLKPA
750
DLTALAKELR
760
AVEDVRPPHK
770
VTDYSSSSEE
780
SGTTDEEDDD
790
VEQEGADEST
800
SGPEDTRAAS
810
SLNLSNGETE
820
SVKTMIVHDD
830
VESEPAMTPS
840
KEGTLIVRQT
850
QSASSTLQKH
860
KSSSSFTPFI
870
DPRLLQISPS
880
SGTTVTSVVG
890
FSCDGMRPEA
900
IRQDPTRKGS
910
VVNVNPTNTR
920
PQSDTPEIRK
930
YKKRFNSEIL
940
CAALWGVNLL
950
VGTESGLMLL
960
DRSGQGKVYP
970
LINRRRFQQM
980
DVLEGLNVLV
990
TISGKKDKLR
1000
VYYLSWLRNK
1010
ILHNDPEVEK
1020
KQGWTTVGDL
1030
EGCVHYKVVK
1040
YERIKFLVIA
1050
LKSSVEVYAW
1060
APKPYHKFMA
1070
FKSFGELVHK
1080
PLLVDLTVEE
1090
GQRLKVIYGS
1100
CAGFHAVDVD
1110
SGSVYDIYLP
1120
THIQCSIKPH
1130
AIIILPNTDG
1140
MELLVCYEDE
1150
GVYVNTYGRI
1160
TKDVVLQWGE
1170
MPTSVAYIRS
1180
NQTMGWGEKA
1190
IEIRSVETGH
1200
LDGVFMHKRA
1210
QRLKFLCERN
1220
DKVFFASVRS
1230
GGSSQVYFMT
LGRTSLLSW
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005925 |
focal adhesion |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0004111 |
creatine kinase activity |
| Molecular Function |
GO:0008349 |
MAP kinase kinase kinase kinase activity |
| Molecular Function |
GO:0008017 |
microtubule binding |
| Molecular Function |
GO:0106310 |
protein serine kinase activity |
| Molecular Function |
GO:0004674 |
protein serine/threonine kinase activity |
| Biological Process |
GO:0035556 |
intracellular signal transduction |
| Biological Process |
GO:0000165 |
MAPK cascade |
| Biological Process |
GO:0043066 |
negative regulation of apoptotic process |
| Biological Process |
GO:0001953 |
negative regulation of cell-matrix adhesion |
| Biological Process |
GO:0048812 |
neuron projection morphogenesis |
| Biological Process |
GO:0032014 |
positive regulation of ARF protein signal transduction |
| Biological Process |
GO:0030335 |
positive regulation of cell migration |
| Biological Process |
GO:0051894 |
positive regulation of focal adhesion assembly |
| Biological Process |
GO:0120183 |
positive regulation of focal adhesion disassembly |
| Biological Process |
GO:0035332 |
positive regulation of hippo signaling |
| Biological Process |
GO:0051549 |
positive regulation of keratinocyte migration |
| Biological Process |
GO:0006468 |
protein phosphorylation |
| Biological Process |
GO:0046328 |
regulation of JNK cascade |
| Biological Process |
GO:0043408 |
regulation of MAPK cascade |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.