Search Results

Overview

Uniprot IDO95831
Protein NameApoptosis-inducing factor 1, mitochondrial
Gene NameAIFM1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
109 LGLTPEQKQKKAALS
112 TPEQKQKKAALSASE
189 SDDPNVTKTLRFKQW
199 RFKQWNGKERSIYFQ
232 VAVLTGKKVVQLDVR
388 IKLKDGRKVETDHIV
518 ATAQDNPKSATEQSG
593 PIARKIIKDGEQHED

Function

Functions both as NADH oxidoreductase and as regulator of apoptosis (PubMed:17094969, PubMed:20362274, PubMed:23217327, PubMed:33168626). In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway (PubMed:20362274). Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains (By similarity). The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (PubMed:20362274). Binds to DNA in a sequence-independent manner (PubMed:27178839). Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis (PubMed:17094969). Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells (PubMed:19418225). In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import (PubMed:26004228)

Protein Sequence

10 MFRCGGLAAG 20 ALKQKLVPLV 30 RTVCVRSPRQ 40 RNRLPGNLFQ 50 RWHVPLELQM 60 TRQMASSGAS 70 GGKIDNSVLV 80 LIVGLSTVGA 90 GAYAYKTMKE 100 DEKRYNERIS 110 GLGLTPEQKQ 120 KKAALSASEG 130 EEVPQDKAPS 140 HVPFLLIGGG 150 TAAFAAARSI 160 RARDPGARVL 170 IVSEDPELPY 180 MRPPLSKELW 190 FSDDPNVTKT 200 LRFKQWNGKE 210 RSIYFQPPSF 220 YVSAQDLPHI 230 ENGGVAVLTG 240 KKVVQLDVRD 250 NMVKLNDGSQ 260 ITYEKCLIAT 270 GGTPRSLSAI 280 DRAGAEVKSR 290 TTLFRKIGDF 300 RSLEKISREV 310 KSITIIGGGF 320 LGSELACALG 330 RKARALGTEV 340 IQLFPEKGNM 350 GKILPEYLSN 360 WTMEKVRREG 370 VKVMPNAIVQ 380 SVGVSSGKLL 390 IKLKDGRKVE 400 TDHIVAAVGL 410 EPNVELAKTG 420 GLEIDSDFGG 430 FRVNAELQAR 440 SNIWVAGDAA 450 CFYDIKLGRR 460 RVEHHDHAVV 470 SGRLAGENMT 480 GAAKPYWHQS 490 MFWSDLGPDV 500 GYEAIGLVDS 510 SLPTVGVFAK 520 ATAQDNPKSA 530 TEQSGTGIRS 540 ESETESEASE 550 ITIPPSTPAV 560 PQAPVQGEDY 570 GKGVIFYLRD 580 KVVVGIVLWN 590 IFNRMPIARK 600 IIKDGEQHED 610 LNEVAKLFNI HED

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005758 mitochondrial intermembrane space
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005634 nucleus
Cellular Component GO:0048471 perinuclear region of cytoplasm
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0071949 FAD binding
Molecular Function GO:0016174 NAD(P)H oxidase H2O2-forming activity
Molecular Function GO:0003954 NADH dehydrogenase activity
Molecular Function GO:0016651 oxidoreductase activity, acting on NAD(P)H
Molecular Function GO:0072572 poly-ADP-D-ribose binding
Molecular Function GO:0046983 protein dimerization activity
Biological Process GO:0006915 apoptotic process
Biological Process GO:1904045 cellular response to aldosterone
Biological Process GO:0071392 cellular response to estradiol stimulus
Biological Process GO:0070301 cellular response to hydrogen peroxide
Biological Process GO:0071456 cellular response to hypoxia
Biological Process GO:0071732 cellular response to nitric oxide
Biological Process GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
Biological Process GO:0160203 mitochondrial disulfide relay system
Biological Process GO:0033108 mitochondrial respiratory chain complex assembly
Biological Process GO:0030182 neuron differentiation
Biological Process GO:0043065 positive regulation of apoptotic process
Biological Process GO:0060545 positive regulation of necroptotic process
Biological Process GO:0043525 positive regulation of neuron apoptotic process
Biological Process GO:0045041 protein import into mitochondrial intermembrane space
Biological Process GO:0002931 response to ischemia
Biological Process GO:1902065 response to L-glutamate
Biological Process GO:0009636 response to toxic substance

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.