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Overview

Uniprot ID	O95865
Protein Name	Putative hydrolase DDAH2
Gene Name	DDAH2
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
194	GSSDAAQKAVRAMAV
267	VSCSELEKAGAGLSS
51	EHGVLGGKLRQRLGL

Function

Putative hydrolase with unknown substrate (Probable). Does not hydrolyze N(G),N(G)-dimethyl-L-arginine (ADMA) which acts as an inhibitor of NOS (PubMed:21493890, PubMed:37296100). In endothelial cells, induces expression of vascular endothelial growth factor (VEGF) via phosphorylation of the transcription factor SP1 by PKA in a process that is independent of NO and NO synthase (By similarity). Similarly, enhances pancreatic insulin secretion through SP1-mediated transcriptional up-regulation of secretagogin/SCGN, an insulin vesicle docking protein (By similarity). Upon viral infection, relocates to mitochondria where it promotes mitochondrial fission through activation of DNM1L leading to the inhibition of innate response activation mediated by MAVS (PubMed:33850055)

Protein Sequence

10 MGTPGEGLGR 20 CSHALIRGVP 30 ESLASGEGAG 40 AGLPALDLAK 50 AQREHGVLGG 60 KLRQRLGLQL 70 LELPPEESLP 80 LGPLLGDTAV 90 IQGDTALITR 100 PWSPARRPEV 110 DGVRKALQDL 120 GLRIVEIGDE 130 NATLDGTDVL 140 FTGREFFVGL 150 SKWTNHRGAE 160 IVADTFRDFA 170 VSTVPVSGPS 180 HLRGLCGMGG 190 PRTVVAGSSD 200 AAQKAVRAMA 210 VLTDHPYASL 220 TLPDDAAADC 230 LFLRPGLPGV 240 PPFLLHRGGG 250 DLPNSQEALQ 260 KLSDVTLVPV 270 SCSELEKAGA 280 GLSSLCLVLS TRPHS

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0016787	hydrolase activity
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0045429	positive regulation of nitric oxide biosynthetic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.