Overview
| Uniprot ID | O95922 |
|---|
| Protein Name | Polyglutamylase complex subunit TTLL1 |
|---|
| Gene Name | TTLL1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 165 |
KKWSRDSKTSSFVSQ |
Function
Catalytic subunit of a polyglutamylase complex which modifies tubulin, generating side chains of glutamate on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin (PubMed:34782749). Probably involved in the side-chain elongation step of the polyglutamylation reaction rather than the initiation step. Modifies both alpha- and beta-tubulins with a preference for the alpha-tail. Unlike most polyglutamylases of the tubulin--tyrosine ligase family, only displays a catalytic activity when in complex with other proteins as it is most likely lacking domains important for autonomous activity. Part of the neuronal tubulin polyglutamylase complex. Mediates cilia and flagella polyglutamylation which is essential for their biogenesis and motility. Involved in respiratory motile cilia function through the regulation of beating asymmetry. Essential for sperm flagella biogenesis, motility and male fertility. Involved in KLF4 glutamylation which impedes its ubiquitination, thereby leading to somatic cell reprogramming, pluripotency maintenance and embryogenesis
Protein Sequence
10
MAGKVKWVTD
20
IEKSVLINNF
30
EKRGWVQVTE
40
NEDWNFYWMS
50
VQTIRNVFSV
60
EAGYRLSDDQ
70
IVNHFPNHYE
80
LTRKDLMVKN
90
IKRYRKELEK
100
EGSPLAEKDE
110
NGKYLYLDFV
120
PVTYMLPADY
130
NLFVEEFRKS
140
PSSTWIMKPC
150
GKAQGKGIFL
160
INKLSQIKKW
170
SRDSKTSSFV
180
SQSNKEAYVI
190
SLYINNPLLI
200
GGRKFDLRLY
210
VLVSTYRPLR
220
CYMYKLGFCR
230
FCTVKYTPST
240
SELDNMFVHL
250
TNVAIQKHGE
260
DYNHIHGGKW
270
TVSNLRLYLE
280
STRGKEVTSK
290
LFDEIHWIIV
300
QSLKAVAPVM
310
NNDKHCFECY
320
GYDIIIDDKL
330
KPWLIEVNAS
340
PSLTSSTAND
350
RILKYNLIND
360
TLNIAVPNGE
370
IPDCKWNKSP
380
PKEVLGNYEI
390
LYDEELAQGD
400
GADRELRSRQ
410
GQSLGPRAGR
420
SRDSGRAVLT
TWK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005930 |
axoneme |
| Cellular Component |
GO:0036064 |
ciliary basal body |
| Cellular Component |
GO:0005874 |
microtubule |
| Cellular Component |
GO:0031514 |
motile cilium |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0106438 |
protein-glutamic acid ligase activity, elongating |
| Molecular Function |
GO:0015631 |
tubulin binding |
| Molecular Function |
GO:0070740 |
tubulin-glutamic acid ligase activity |
| Biological Process |
GO:0000226 |
microtubule cytoskeleton organization |
| Biological Process |
GO:0018095 |
protein polyglutamylation |
| Biological Process |
GO:0007288 |
sperm axoneme assembly |
Reference
[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.