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Overview

Uniprot IDO96006
Protein NameE3 SUMO-protein ligase ZBED1
Gene NameZBED1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
96 AFATAFSKLKPESSQ

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase which sumoylates CHD3/Mi2-alpha, causing its release from DNA (PubMed:27068747). This results in suppression of CHD3/Mi2-alpha transcription repression, increased recruitment of RNA polymerase II to gene promoters and positive regulation of transcription including H1-5 and ribosomal proteins such as: RPS6, RPL10A, and RPL12 (PubMed:12663651, PubMed:17209048, PubMed:17220279, PubMed:27068747). The resulting increased transcriptional activity drives cell proliferation (PubMed:12663651, PubMed:17220279). Binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in gene promoters of ribosomal proteins (PubMed:12663651, PubMed:17209048, PubMed:17220279, PubMed:27068747)

Protein Sequence

10 MENKSLESSQ 20 TDLKLVAHPR 30 AKSKVWKYFG 40 FDTNAEGCIL 50 QWKKIYCRIC 60 MAQIAYSGNT 70 SNLSYHLEKN 80 HPEEFCEFVK 90 SNTEQMREAF 100 ATAFSKLKPE 110 SSQQPGQDAL 120 AVKAGHGYDS 130 KKQQELTAAV 140 LGLICEGLYP 150 ASIVDEPTFK 160 VLLKTADPRY 170 ELPSRKYIST 180 KAIPEKYGAV 190 REVILKELAE 200 ATWCGISTDM 210 WRSENQNRAY 220 VTLAAHFLGL 230 GAPNCLSMGS 240 RCLKTFEVPE 250 ENTAETITRV 260 LYEVFIEWGI 270 SAKVFGATTN 280 YGKDIVKACS 290 LLDVAVHMPC 300 LGHTFNAGIQ 310 QAFQLPKLGA 320 LLSRCRKLVE 330 YFQQSAVAMY 340 MLYEKQKQQN 350 VAHCMLVSNR 360 VSWWGSTLAM 370 LQRLKEQQFV 380 IAGVLVEDSN 390 NHHLMLEASE 400 WATIEGLVEL 410 LQPFKQVAEM 420 LSASRYPTIS 430 MVKPLLHMLL 440 NTTLNIKETD 450 SKELSMAKEV 460 IAKELSKTYQ 470 ETPEIDMFLN 480 VATFLDPRYK 490 RLPFLSAFER 500 QQVENRVVEE 510 AKGLLDKVKD 520 GGYRPAEDKI 530 FPVPEEPPVK 540 KLMRTSTPPP 550 ASVINNMLAE 560 IFCQTGGVED 570 QEEWHAQVVE 580 ELSNFKSQKV 590 LGLNEDPLKW 600 WSDRLALFPL 610 LPKVLQKYWC 620 VTATRVAPER 630 LFGSAANVVS 640 AKRNRLAPAH 650 VDEQVFLYEN 660 ARSGAEAEPE 670 DQDEGEWGLD 680 QEQVFSLGDG 690 VSGGFFGIRD SSFL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0031965 nuclear membrane
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0016605 PML body
Molecular Function GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
Molecular Function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0046983 protein dimerization activity
Molecular Function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function GO:0043565 sequence-specific DNA binding
Molecular Function GO:1990837 sequence-specific double-stranded DNA binding
Molecular Function GO:0061665 SUMO ligase activity
Molecular Function GO:0019789 SUMO transferase activity
Molecular Function GO:0003713 transcription coactivator activity
Molecular Function GO:0003712 transcription coregulator activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0044828 host-mediated suppression of viral genome replication
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:1990466 protein autosumoylation
Biological Process GO:0016925 protein sumoylation
Biological Process GO:0006355 regulation of DNA-templated transcription
Biological Process GO:0006357 regulation of transcription by RNA polymerase II

Reference

PMID: N/A