Search Results

Overview

Uniprot IDO96028
Protein NameHistone-lysine N-methyltransferase NSD2
Gene NameNSD2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
17 LSVQSVVKCIKMKQA
404 MKRRRRAKLCSSAET
453 VSMPRSRKGDAASQF
505 QRARYNTKFALVAPV
524 DSGNVNGKKRNHTKR
525 SGNVNGKKRNHTKRI
565 KRDTITDKTARTSSY
573 TARTSSYKAMEAASS
582 MEAASSLKSQAATKN
598 SDACKPLKKRNRAST
995 PPPYKHIKVNKPYGK

Function

Histone methyltransferase which specifically dimethylates nucleosomal histone H3 at 'Lys-36' (H3K36me2) (PubMed:19808676, PubMed:22099308, PubMed:27571355, PubMed:29728617, PubMed:33941880). Also monomethylates nucleosomal histone H3 at 'Lys-36' (H3K36me) in vitro (PubMed:22099308). Does not trimethylate nucleosomal histone H3 at 'Lys-36' (H3K36me3) (PubMed:22099308). However, specifically trimethylates histone H3 at 'Lys-36' (H3K36me3) at euchromatic regions in embryonic stem (ES) cells (By similarity). By methylating histone H3 at 'Lys-36', involved in the regulation of gene transcription during various biological processes (PubMed:16115125, PubMed:22099308, PubMed:29728617). In ES cells, associates with developmental transcription factors such as SALL1 and represses inappropriate gene transcription mediated by histone deacetylation (By similarity). During heart development, associates with transcription factor NKX2-5 to repress transcription of NKX2-5 target genes (By similarity). Plays an essential role in adipogenesis, by regulating expression of genes involved in pre-adipocyte differentiation (PubMed:29728617). During T-cell receptor (TCR) and CD28-mediated T-cell activation, promotes the transcription of transcription factor BCL6 which is required for follicular helper T (Tfh) cell differentiation (By similarity). During B-cell development, required for the generation of the B1 lineage (By similarity). During B2 cell activation, may contribute to the control of isotype class switch recombination (CRS), splenic germinal center formation, and the humoral immune response (By similarity). Plays a role in class switch recombination of the immunoglobulin heavy chain (IgH) locus during B-cell activation (By similarity). By regulating the methylation of histone H3 at 'Lys-36' and histone H4 at 'Lys-20' at the IgH locus, involved in TP53BP1 recruitment to the IgH switch region and promotes the transcription of IgA (By similarity)

Protein Sequence

10 MEFSIKQSPL 20 SVQSVVKCIK 30 MKQAPEILGS 40 ANGKTPSCEV 50 NRECSVFLSK 60 AQLSSSLQEG 70 VMQKFNGHDA 80 LPFIPADKLK 90 DLTSRVFNGE 100 PGAHDAKLRF 110 ESQEMKGIGT 120 PPNTTPIKNG 130 SPEIKLKITK 140 TYMNGKPLFE 150 SSICGDSAAD 160 VSQSEENGQK 170 PENKARRNRK 180 RSIKYDSLLE 190 QGLVEAALVS 200 KISSPSDKKI 210 PAKKESCPNT 220 GRDKDHLLKY 230 NVGDLVWSKV 240 SGYPWWPCMV 250 SADPLLHSYT 260 KLKGQKKSAR 270 QYHVQFFGDA 280 PERAWIFEKS 290 LVAFEGEGQF 300 EKLCQESAKQ 310 APTKAEKIKL 320 LKPISGKLRA 330 QWEMGIVQAE 340 EAASMSVEER 350 KAKFTFLYVG 360 DQLHLNPQVA 370 KEAGIAAESL 380 GEMAESSGVS 390 EEAAENPKSV 400 REECIPMKRR 410 RRAKLCSSAE 420 TLESHPDIGK 430 STPQKTAEAD 440 PRRGVGSPPG 450 RKKTTVSMPR 460 SRKGDAASQF 470 LVFCQKHRDE 480 VVAEHPDASG 490 EEIEELLRSQ 500 WSLLSEKQRA 510 RYNTKFALVA 520 PVQAEEDSGN 530 VNGKKRNHTK 540 RIQDPTEDAE 550 AEDTPRKRLR 560 TDKHSLRKRD 570 TITDKTARTS 580 SYKAMEAASS 590 LKSQAATKNL 600 SDACKPLKKR 610 NRASTAASSA 620 LGFSKSSSPS 630 ASLTENEVSD 640 SPGDEPSESP 650 YESADETQTE 660 VSVSSKKSER 670 GVTAKKEYVC 680 QLCEKPGSLL 690 LCEGPCCGAF 700 HLACLGLSRR 710 PEGRFTCSEC 720 ASGIHSCFVC 730 KESKTDVKRC 740 VVTQCGKFYH 750 EACVKKYPLT 760 VFESRGFRCP 770 LHSCVSCHAS 780 NPSNPRPSKG 790 KMMRCVRCPV 800 AYHSGDACLA 810 AGCSVIASNS 820 IICTAHFTAR 830 KGKRHHAHVN 840 VSWCFVCSKG 850 GSLLCCESCP 860 AAFHPDCLNI 870 EMPDGSWFCN 880 DCRAGKKLHF 890 QDIIWVKLGN 900 YRWWPAEVCH 910 PKNVPPNIQK 920 MKHEIGEFPV 930 FFFGSKDYYW 940 THQARVFPYM 950 EGDRGSRYQG 960 VRGIGRVFKN 970 ALQEAEARFR 980 EIKLQREARE 990 TQESERKPPP 1000 YKHIKVNKPY 1010 GKVQIYTADI 1020 SEIPKCNCKP 1030 TDENPCGFDS 1040 ECLNRMLMFE 1050 CHPQVCPAGE 1060 FCQNQCFTKR 1070 QYPETKIIKT 1080 DGKGWGLVAK 1090 RDIRKGEFVN 1100 EYVGELIDEE 1110 ECMARIKHAH 1120 ENDITHFYML 1130 TIDKDRIIDA 1140 GPKGNYSRFM 1150 NHSCQPNCET 1160 LKWTVNGDTR 1170 VGLFAVCDIP 1180 AGTELTFNYN 1190 LDCLGNEKTV 1200 CRCGASNCSG 1210 FLGDRPKTST 1220 TLSSEEKGKK 1230 TKKKTRRRRA 1240 KGEGKRQSED 1250 ECFRCGDGGQ 1260 LVLCDRKFCT 1270 KAYHLSCLGL 1280 GKRPFGKWEC 1290 PWHHCDVCGK 1300 PSTSFCHLCP 1310 NSFCKEHQDG 1320 TAFSCTPDGR 1330 SYCCEHDLGA 1340 ASVRSTKTEK 1350 PPPEPGKPKG 1360 KRRRRRGWRR VTEGK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0140938 histone H3 methyltransferase activity
Molecular Function GO:0140954 histone H3K36 dimethyltransferase activity
Molecular Function GO:0046975 histone H3K36 methyltransferase activity
Molecular Function GO:0140955 histone H3K36 trimethyltransferase activity
Molecular Function GO:0042799 histone H4K20 methyltransferase activity
Molecular Function GO:0043565 sequence-specific DNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0006302 double-strand break repair
Biological Process GO:0032259 methylation
Biological Process GO:0006355 regulation of DNA-templated transcription

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.