Overview
| Uniprot ID | P00346 |
|---|
| Protein Name | Malate dehydrogenase, mitochondrial |
|---|
| Gene Name | MDH2 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 157 |
ITAEVFKKHGVYNPN |
| 185 |
NAFVAELKGLDPARV |
| 239 |
EAGTEVVKAKAGAGS |
| 301 |
LGKKGIEKNLGIGKI |
| 307 |
EKNLGIGKISPFEEK |
| 329 |
ELKASIKKGEEFVKN |
| 78 |
IETRATVKGYLGPEQ |
Function
Mitochondrial malate dehydrogenase that catalyzes the reversible conversion of malate to oxaloacetate using NAD(+) as a cofactor. Plays a central role in the tricarboxylic acid (TCA) cycle contributing to cellular respiration and energy production via the mitochondrial electron transport chain (By similarity). Also participates in the malate-aspartate NADH shuttle, facilitating transfer of reducing equivalents between cytosol and mitochondria to maintain redox balance (NAD(+)/NADH homeostasis) and support metabolic flux between compartments (By similarity). In addition to its metabolic role, binds to RNA in cells, associating with cytosolic RNAs including tRNAs and mRNAs (By similarity)
Protein Sequence
10
MLSALARPAG
20
AALRRSFSTS
30
XQNNAKVAVL
40
GASGGIGQPL
50
SLLLKNSPLV
60
SRLTLYDIAH
70
TPGVAADLSH
80
IETRATVKGY
90
LGPEQLPDCL
100
KGCDVVVIPA
110
GVPRKPGMTR
120
DDLFNTNATI
130
VATLTAACAQ
140
HCPDAMICII
150
SNPVNSTIPI
160
TAEVFKKHGV
170
YNPNKIFGVT
180
TLDIVRANAF
190
VAELKGLDPA
200
RVSVPVIGGH
210
AGKTIIPLIS
220
QCTPKVDFPQ
230
DQLSTLTGRI
240
QEAGTEVVKA
250
KAGAGSATLS
260
MAYAGARFVF
270
SLVDAMNGKE
280
GVVECSFVKS
290
QETDCPYFST
300
PLLLGKKGIE
310
KNLGIGKISP
320
FEEKMIAEAI
330
PELKASIKKG
EEFVKNMK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Molecular Function |
GO:0030060 |
L-malate dehydrogenase (NAD+) activity |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Molecular Function |
GO:0051087 |
protein-folding chaperone binding |
| Biological Process |
GO:0009060 |
aerobic respiration |
| Biological Process |
GO:0006108 |
malate metabolic process |
| Biological Process |
GO:0006099 |
tricarboxylic acid cycle |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.