Overview
| Uniprot ID | P00355 |
|---|
| Protein Name | Glyceraldehyde-3-phosphate dehydrogenase |
|---|
| Gene Name | GAPDH |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 111 |
EKAGAHLKGGAKRVI |
| 143 |
EKYDNSLKIVSNASC |
| 192 |
TVDGPSGKLWRDGRG |
| 213 |
PASTGAAKAVGKVIP |
| 217 |
GAAKAVGKVIPELNG |
| 252 |
CRLEKPAKYDDIKKV |
| 261 |
DDIKKVVKQASEGPL |
| 3 |
*****MVKVGVNGFG |
| 59 |
GKFHGTVKAENGKLV |
| 64 |
TVKAENGKLVINGKA |
Function
Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions (By similarity). Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity)
Protein Sequence
10
MVKVGVNGFG
20
RIGRLVTRAA
30
FNSGKVDIVA
40
INDPFIDLHY
50
MVYMFQYDST
60
HGKFHGTVKA
70
ENGKLVINGK
80
AITIFQERDP
90
ANIKWGDAGA
100
TYVVESTGVF
110
TTMEKAGAHL
120
KGGAKRVIIS
130
APSADAPMFV
140
MGVNHEKYDN
150
SLKIVSNASC
160
TTNCLAPLAK
170
VIHDHFGIVE
180
GLMTTVHAIT
190
ATQKTVDGPS
200
GKLWRDGRGA
210
AQNIIPASTG
220
AAKAVGKVIP
230
ELNGKLTGMA
240
FRVPTPNVSV
250
VDLTCRLEKP
260
AKYDDIKKVV
270
KQASEGPLKG
280
ILGYTEDQVV
290
SCDFNSDTHS
300
STFDAGAGIA
310
LNDHFVKLIS
320
WYDNEFGYSN
330
RVVDLMVHMA
SKE
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0097452 |
GAIT complex |
| Cellular Component |
GO:0005811 |
lipid droplet |
| Cellular Component |
GO:0015630 |
microtubule cytoskeleton |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:1990904 |
ribonucleoprotein complex |
| Molecular Function |
GO:0019828 |
aspartic-type endopeptidase inhibitor activity |
| Molecular Function |
GO:0097718 |
disordered domain specific binding |
| Molecular Function |
GO:0004365 |
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0008017 |
microtubule binding |
| Molecular Function |
GO:0051287 |
NAD binding |
| Molecular Function |
GO:0050661 |
NADP binding |
| Molecular Function |
GO:0035605 |
peptidyl-cysteine S-nitrosylase activity |
| Biological Process |
GO:0061844 |
antimicrobial humoral immune response mediated by antimicrobial peptide |
| Biological Process |
GO:0061621 |
canonical glycolysis |
| Biological Process |
GO:0071346 |
cellular response to type II interferon |
| Biological Process |
GO:0050832 |
defense response to fungus |
| Biological Process |
GO:0051873 |
killing by host of symbiont cells |
| Biological Process |
GO:0000226 |
microtubule cytoskeleton organization |
| Biological Process |
GO:0017148 |
negative regulation of translation |
| Biological Process |
GO:0051402 |
neuron apoptotic process |
| Biological Process |
GO:0035606 |
peptidyl-cysteine S-trans-nitrosylation |
| Biological Process |
GO:0043123 |
positive regulation of canonical NF-kappaB signal transduction |
| Biological Process |
GO:0032481 |
positive regulation of type I interferon production |
| Biological Process |
GO:0050821 |
protein stabilization |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.