Search Results

Overview

Uniprot IDP00416
Protein NameCytochrome c oxidase subunit 3
Gene Namemt-Co3
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
224 VCLLRQLKFHFTSKH

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation (PubMed:34616041, PubMed:38575788). The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase (PubMed:34616041, PubMed:38575788). Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water (PubMed:34616041). Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)) (PubMed:34616041). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix (PubMed:34616041)

Protein Sequence

10 MTHQTHAYHM 20 VNPSPWPLTG 30 AFSALLLTSG 40 LVMWFHYNSI 50 TLLTLGLLTN 60 ILTMYQWWRD 70 VIREGTYQGH 80 HTPIVQKGLR 90 YGMILFIVSE 100 VFFFAGFFWA 110 FYHSSLVPTH 120 DLGGCWPPTG 130 ISPLNPLEVP 140 LLNTSVLLAS 150 GVSITWAHHS 160 LMEGKRNHMN 170 QALLITIMLG 180 LYFTILQASE 190 YFETSFSISD 200 GIYGSTFFMA 210 TGFHGLHVII 220 GSTFLIVCLL 230 RQLKFHFTSK 240 HHFGFEAAAW 250 YWHFVDVVWL 260 FLYVSIYWWG S

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0031966 mitochondrial membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0045277 respiratory chain complex IV
Molecular Function GO:0004129 cytochrome-c oxidase activity
Biological Process GO:0006123 mitochondrial electron transport, cytochrome c to oxygen
Biological Process GO:0008535 respiratory chain complex IV assembly

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.