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Overview

Uniprot IDP00734
Protein NameProthrombin
Gene NameF2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
247 AQAKALSKHQDFNSA
498 SLLQAGYKGRVTGWG
537 IVERPVCKDSTRIRI

Function

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (PubMed:2019570, PubMed:21976677). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (PubMed:30568593, PubMed:9780208)

Protein Sequence

10 MAHVRGLQLP 20 GCLALAALCS 30 LVHSQHVFLA 40 PQQARSLLQR 50 VRRANTFLEE 60 VRKGNLEREC 70 VEETCSYEEA 80 FEALESSTAT 90 DVFWAKYTAC 100 ETARTPRDKL 110 AACLEGNCAE 120 GLGTNYRGHV 130 NITRSGIECQ 140 LWRSRYPHKP 150 EINSTTHPGA 160 DLQENFCRNP 170 DSSTTGPWCY 180 TTDPTVRRQE 190 CSIPVCGQDQ 200 VTVAMTPRSE 210 GSSVNLSPPL 220 EQCVPDRGQQ 230 YQGRLAVTTH 240 GLPCLAWASA 250 QAKALSKHQD 260 FNSAVQLVEN 270 FCRNPDGDEE 280 GVWCYVAGKP 290 GDFGYCDLNY 300 CEEAVEEETG 310 DGLDEDSDRA 320 IEGRTATSEY 330 QTFFNPRTFG 340 SGEADCGLRP 350 LFEKKSLEDK 360 TERELLESYI 370 DGRIVEGSDA 380 EIGMSPWQVM 390 LFRKSPQELL 400 CGASLISDRW 410 VLTAAHCLLY 420 PPWDKNFTEN 430 DLLVRIGKHS 440 RTRYERNIEK 450 ISMLEKIYIH 460 PRYNWRENLD 470 RDIALMKLKK 480 PVAFSDYIHP 490 VCLPDRETAA 500 SLLQAGYKGR 510 VTGWGNLKET 520 WTANVGKGQP 530 SVLQVVNLPI 540 VERPVCKDST 550 RIRITDNMFC 560 AGYKPDEGKR 570 GDACEGDSGG 580 PFVMKSPFNN 590 RWYQMGIVSW 600 GEGCDRDGKY 610 GFYTHVFRLK 620 KWIQKVIDQF GE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0072562 blood microparticle
Cellular Component GO:0005788 endoplasmic reticulum lumen
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0031012 extracellular matrix
Cellular Component GO:0005576 extracellular region
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0005796 Golgi lumen
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0005509 calcium ion binding
Molecular Function GO:0008083 growth factor activity
Molecular Function GO:0008201 heparin binding
Molecular Function GO:0001530 lipopolysaccharide binding
Molecular Function GO:0048018 receptor ligand activity
Molecular Function GO:0004252 serine-type endopeptidase activity
Molecular Function GO:0005102 signaling receptor binding
Molecular Function GO:0070053 thrombospondin receptor activity
Biological Process GO:0006953 acute-phase response
Biological Process GO:0061844 antimicrobial humoral immune response mediated by antimicrobial peptide
Biological Process GO:0007596 blood coagulation
Biological Process GO:0007166 cell surface receptor signaling pathway
Biological Process GO:0051838 cytolysis by host of symbiont cells
Biological Process GO:0042730 fibrinolysis
Biological Process GO:0048712 negative regulation of astrocyte differentiation
Biological Process GO:0030195 negative regulation of blood coagulation
Biological Process GO:1900016 negative regulation of cytokine production involved in inflammatory response
Biological Process GO:0051918 negative regulation of fibrinolysis
Biological Process GO:0010544 negative regulation of platelet activation
Biological Process GO:0045861 negative regulation of proteolysis
Biological Process GO:0070945 neutrophil-mediated killing of gram-negative bacterium
Biological Process GO:0030168 platelet activation
Biological Process GO:0030194 positive regulation of blood coagulation
Biological Process GO:0032967 positive regulation of collagen biosynthetic process
Biological Process GO:1900738 positive regulation of phospholipase C-activating G protein-coupled receptor signaling pathway
Biological Process GO:1900182 positive regulation of protein localization to nucleus
Biological Process GO:2000379 positive regulation of reactive oxygen species metabolic process
Biological Process GO:0046427 positive regulation of receptor signaling pathway via JAK-STAT
Biological Process GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
Biological Process GO:0006508 proteolysis
Biological Process GO:0030193 regulation of blood coagulation
Biological Process GO:0051480 regulation of cytosolic calcium ion concentration
Biological Process GO:0009611 response to wounding
Biological Process GO:0070493 thrombin-activated receptor signaling pathway

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.