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Overview

Uniprot IDP00966
Protein NameArgininosuccinate synthase
Gene NameASS1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
101 ARPCIARKQVEIAQR
112 IAQREGAKYVSHGAT
121 VSHGATGKGNDQVRF
165 NDLMEYAKQHGIPIP
209 APPGLYTKTQDPAKA
215 TKTQDPAKAPNTPDI
228 DILEIEFKKGVPVKV
234 FKKGVPVKVTNVKDG
277 ENRFIGMKSRGIYET
310 DREVRKIKQGLGLKF
340 FVRHCIAKSQERVEG
348 SQERVEGKVQVSVLK
355 KVQVSVLKGQVYILG
408 EYHRLQSKVTAK***
53 EARKKALKLGAKKVF
58 ALKLGAKKVFIEDVS

Function

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues

Protein Sequence

10 MSSKGSVVLA 20 YSGGLDTSCI 30 LVWLKEQGYD 40 VIAYLANIGQ 50 KEDFEEARKK 60 ALKLGAKKVF 70 IEDVSREFVE 80 EFIWPAIQSS 90 ALYEDRYLLG 100 TSLARPCIAR 110 KQVEIAQREG 120 AKYVSHGATG 130 KGNDQVRFEL 140 SCYSLAPQIK 150 VIAPWRMPEF 160 YNRFKGRNDL 170 MEYAKQHGIP 180 IPVTPKNPWS 190 MDENLMHISY 200 EAGILENPKN 210 QAPPGLYTKT 220 QDPAKAPNTP 230 DILEIEFKKG 240 VPVKVTNVKD 250 GTTHQTSLEL 260 FMYLNEVAGK 270 HGVGRIDIVE 280 NRFIGMKSRG 290 IYETPAGTIL 300 YHAHLDIEAF 310 TMDREVRKIK 320 QGLGLKFAEL 330 VYTGFWHSPE 340 CEFVRHCIAK 350 SQERVEGKVQ 360 VSVLKGQVYI 370 LGRESPLSLY 380 NEELVSMNVQ 390 GDYEPTDATG 400 FININSLRLK 410 EYHRLQSKVT AK

Gene Ontology

Classification GO ID Description
Biological Process GO:0000053 argininosuccinate metabolic process
Cellular Component GO:0070852 cell body fiber
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005741 mitochondrial outer membrane
Cellular Component GO:0043204 perikaryon
Molecular Function GO:0016597 amino acid binding
Molecular Function GO:0004055 argininosuccinate synthase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0015643 toxic substance binding
Biological Process GO:0006953 acute-phase response
Biological Process GO:0006531 aspartate metabolic process
Biological Process GO:0071418 cellular response to amine stimulus
Biological Process GO:0071230 cellular response to amino acid stimulus
Biological Process GO:0071242 cellular response to ammonium ion
Biological Process GO:0071320 cellular response to cAMP
Biological Process GO:0071549 cellular response to dexamethasone stimulus
Biological Process GO:0071377 cellular response to glucagon stimulus
Biological Process GO:0071499 cellular response to laminar fluid shear stress
Biological Process GO:0071222 cellular response to lipopolysaccharide
Biological Process GO:0071400 cellular response to oleic acid
Biological Process GO:0071356 cellular response to tumor necrosis factor
Biological Process GO:0071346 cellular response to type II interferon
Biological Process GO:0007623 circadian rhythm
Biological Process GO:0000052 citrulline metabolic process
Biological Process GO:0060539 diaphragm development
Biological Process GO:0001822 kidney development
Biological Process GO:0006526 L-arginine biosynthetic process
Biological Process GO:0001889 liver development
Biological Process GO:0007494 midgut development
Biological Process GO:1903038 negative regulation of leukocyte cell-cell adhesion
Biological Process GO:0045429 positive regulation of nitric oxide biosynthetic process
Biological Process GO:0032355 response to estradiol
Biological Process GO:0060416 response to growth hormone
Biological Process GO:0010046 response to mycotoxin
Biological Process GO:0007584 response to nutrient
Biological Process GO:0009410 response to xenobiotic stimulus
Biological Process GO:0010043 response to zinc ion
Biological Process GO:0000050 urea cycle

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.