Search Results

Overview

Uniprot IDP04424
Protein NameArgininosuccinate lyase
Gene NameASL
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
106 LIGATAGKLHTGRSR
288 SSLMPQKKNPDSLEL
393 QAHEASGKAVFMAET
51 AYSRGLEKAGLLTKA
7 *MASESGKLWGGRFV
97 TANERRLKELIGATA

Function

Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues (PubMed:11747432, PubMed:11747433, PubMed:22081021, PubMed:2263616, PubMed:9045711). Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway (PubMed:22081021)

Protein Sequence

10 MASESGKLWG 20 GRFVGAVDPI 30 MEKFNASIAY 40 DRHLWEVDVQ 50 GSKAYSRGLE 60 KAGLLTKAEM 70 DQILHGLDKV 80 AEEWAQGTFK 90 LNSNDEDIHT 100 ANERRLKELI 110 GATAGKLHTG 120 RSRNDQVVTD 130 LRLWMRQTCS 140 TLSGLLWELI 150 RTMVDRAEAE 160 RDVLFPGYTH 170 LQRAQPIRWS 180 HWILSHAVAL 190 TRDSERLLEV 200 RKRINVLPLG 210 SGAIAGNPLG 220 VDRELLRAEL 230 NFGAITLNSM 240 DATSERDFVA 250 EFLFWASLCM 260 THLSRMAEDL 270 ILYCTKEFSF 280 VQLSDAYSTG 290 SSLMPQKKNP 300 DSLELIRSKA 310 GRVFGRCAGL 320 LMTLKGLPST 330 YNKDLQEDKE 340 AVFEVSDTMS 350 AVLQVATGVI 360 STLQIHQENM 370 GQALSPDMLA 380 TDLAYYLVRK 390 GMPFRQAHEA 400 SGKAVFMAET 410 KGVALNQLSL 420 QELQTISPLF 430 SGDVICVWDY 440 GHSVEQYGAL 450 GGTARSSVDW 460 QIRQVRALLQ AQQA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Molecular Function GO:0004056 argininosuccinate lyase activity
Molecular Function GO:0042802 identical protein binding
Biological Process GO:0006525 arginine metabolic process
Biological Process GO:0006526 L-arginine biosynthetic process
Biological Process GO:0042450 L-arginine biosynthetic process via ornithine
Biological Process GO:0045429 positive regulation of nitric oxide biosynthetic process
Biological Process GO:0000050 urea cycle

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.