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Overview

Uniprot IDP04636
Protein NameMalate dehydrogenase, mitochondrial
Gene NameMdh2
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
157 ITAEVFKKHGVYNPN
215 LISQCTPKVDFPQDQ
239 EAGTEVVKAKAGAGS
241 GTEVVKAKAGAGSAT
296 STPLLLGKKGLEKNL
301 LGKKGLEKNLGIGKI
307 EKNLGIGKITPFEEK
314 KITPFEEKMIAEAIP
324 AEAIPELKASIKKGE
335 KKGEDFVKNMK****
45 QPLSLLLKNSPLVSR
78 IETRANVKGYLGPEQ

Function

Mitochondrial malate dehydrogenase that catalyzes the reversible conversion of malate to oxaloacetate using NAD(+) as a cofactor. Plays a central role in the tricarboxylic acid (TCA) cycle contributing to cellular respiration and energy production via the mitochondrial electron transport chain (By similarity). Also participates in the malate-aspartate NADH shuttle, facilitating transfer of reducing equivalents between cytosol and mitochondria to maintain redox balance (NAD(+)/NADH homeostasis) and support metabolic flux between compartments (By similarity). In addition to its metabolic role, binds to RNA in cells, associating with cytosolic RNAs including tRNAs and mRNAs (By similarity)

Protein Sequence

10 MLSALARPVG 20 AALRRSFSTS 30 AQNNAKVAVL 40 GASGGIGQPL 50 SLLLKNSPLV 60 SRLTLYDIAH 70 TPGVAADLSH 80 IETRANVKGY 90 LGPEQLPDCL 100 KGCDVVVIPA 110 GVPRKPGMTR 120 DDLFNTNATI 130 VATLTAACAQ 140 HCPEAMICII 150 SNPVNSTIPI 160 TAEVFKKHGV 170 YNPNKIFGVT 180 TLDIVRANTF 190 VAELKGLDPA 200 RVNVPVIGGH 210 AGKTIIPLIS 220 QCTPKVDFPQ 230 DQLATLTGRI 240 QEAGTEVVKA 250 KAGAGSATLS 260 MAYAGARFVF 270 SLVDAMNGKE 280 GVIECSFVQS 290 KETECTYFST 300 PLLLGKKGLE 310 KNLGIGKITP 320 FEEKMIAEAI 330 PELKASIKKG EDFVKNMK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0016020 membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0030060 L-malate dehydrogenase (NAD+) activity
Molecular Function GO:0046554 L-malate dehydrogenase (NADP+) activity
Molecular Function GO:0016615 malate dehydrogenase activity
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0051087 protein-folding chaperone binding
Biological Process GO:0009060 aerobic respiration
Biological Process GO:0006094 gluconeogenesis
Biological Process GO:0006108 malate metabolic process
Biological Process GO:0043490 malate-aspartate shuttle
Biological Process GO:0006099 tricarboxylic acid cycle

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[3] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.