Overview
| Uniprot ID | P04797 |
|---|
| Protein Name | Glyceraldehyde-3-phosphate dehydrogenase |
|---|
| Gene Name | Gapdh |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 192 |
TVDGPSGKLWRDGRG |
| 213 |
PASTGAAKAVGKVIP |
| 261 |
DDIKKVVKQAAEGPL |
Function
Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (PubMed:17934141). Also shows nitrosylase activity, thereby playing a role in nuclear functions (PubMed:15951807, PubMed:20972425). Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (PubMed:15312048). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (PubMed:10424669, PubMed:15951807, PubMed:20972425). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (PubMed:15951807, PubMed:20972425)
Protein Sequence
10
MVKVGVNGFG
20
RIGRLVTRAA
30
FSCDKVDIVA
40
INDPFIDLNY
50
MVYMFQYDST
60
HGKFNGTVKA
70
ENGKLVINGK
80
PITIFQERDP
90
ANIKWGDAGA
100
EYVVESTGVF
110
TTMEKAGAHL
120
KGGAKRVIIS
130
APSADAPMFV
140
MGVNHEKYDN
150
SLKIVSNASC
160
TTNCLAPLAK
170
VIHDNFGIVE
180
GLMTTVHAIT
190
ATQKTVDGPS
200
GKLWRDGRGA
210
AQNIIPASTG
220
AAKAVGKVIP
230
ELNGKLTGMA
240
FRVPTPNVSV
250
VDLTCRLEKP
260
AKYDDIKKVV
270
KQAAEGPLKG
280
ILGYTEDQVV
290
SCDFNSNSHS
300
STFDAGAGIA
310
LNDNFVKLIS
320
WYDNEYGYSN
330
RVVDLMAYMA
SKE
Gene Ontology
| Classification |
GO ID |
Description |
| Molecular Function |
GO:0019828 |
aspartic-type endopeptidase inhibitor activity |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0097452 |
GAIT complex |
| Cellular Component |
GO:0098978 |
glutamatergic synapse |
| Cellular Component |
GO:0005811 |
lipid droplet |
| Cellular Component |
GO:0015630 |
microtubule cytoskeleton |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0099092 |
postsynaptic density, intracellular component |
| Cellular Component |
GO:1990904 |
ribonucleoprotein complex |
| Molecular Function |
GO:0097718 |
disordered domain specific binding |
| Molecular Function |
GO:0019899 |
enzyme binding |
| Molecular Function |
GO:0004365 |
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0008017 |
microtubule binding |
| Molecular Function |
GO:0051287 |
NAD binding |
| Molecular Function |
GO:0050661 |
NADP binding |
| Molecular Function |
GO:0035605 |
peptidyl-cysteine S-nitrosylase activity |
| Biological Process |
GO:0061844 |
antimicrobial humoral immune response mediated by antimicrobial peptide |
| Biological Process |
GO:0141156 |
cAMP/PKA signal transduction |
| Biological Process |
GO:0061621 |
canonical glycolysis |
| Biological Process |
GO:0071346 |
cellular response to type II interferon |
| Biological Process |
GO:0007565 |
female pregnancy |
| Biological Process |
GO:0006094 |
gluconeogenesis |
| Biological Process |
GO:0006096 |
glycolytic process |
| Biological Process |
GO:0000226 |
microtubule cytoskeleton organization |
| Biological Process |
GO:0010951 |
negative regulation of endopeptidase activity |
| Biological Process |
GO:0017148 |
negative regulation of translation |
| Biological Process |
GO:1905460 |
negative regulation of vascular associated smooth muscle cell apoptotic process |
| Biological Process |
GO:0051402 |
neuron apoptotic process |
| Biological Process |
GO:0007263 |
nitric oxide mediated signal transduction |
| Biological Process |
GO:0035606 |
peptidyl-cysteine S-trans-nitrosylation |
| Biological Process |
GO:0043123 |
positive regulation of canonical NF-kappaB signal transduction |
| Biological Process |
GO:0001819 |
positive regulation of cytokine production |
| Biological Process |
GO:0032481 |
positive regulation of type I interferon production |
| Biological Process |
GO:0050821 |
protein stabilization |
| Biological Process |
GO:0045471 |
response to ethanol |
Reference
[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.