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Overview

Uniprot IDP04818
Protein NameThymidylate synthase
Gene NameTYMS
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
77 EFPLLTTKRVFWKGV

Function

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the mitochondrial and nuclear de novo thymidylate biosynthesis pathway

Protein Sequence

10 MPVAGSELPR 20 RPLPPAAQER 30 DAEPRPPHGE 40 LQYLGQIQHI 50 LRCGVRKDDR 60 TGTGTLSVFG 70 MQARYSLRDE 80 FPLLTTKRVF 90 WKGVLEELLW 100 FIKGSTNAKE 110 LSSKGVKIWD 120 ANGSRDFLDS 130 LGFSTREEGD 140 LGPVYGFQWR 150 HFGAEYRDME 160 SDYSGQGVDQ 170 LQRVIDTIKT 180 NPDDRRIIMC 190 AWNPRDLPLM 200 ALPPCHALCQ 210 FYVVNSELSC 220 QLYQRSGDMG 230 LGVPFNIASY 240 ALLTYMIAHI 250 TGLKPGDFIH 260 TLGDAHIYLN 270 HIEPLKIQLQ 280 REPRPFPKLR 290 ILRKVEKIDD 300 FKAEDFQIEG 310 YNPHPTIKME MAV

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005657 replication fork
Molecular Function GO:0005542 folic acid binding
Molecular Function GO:0000900 mRNA regulatory element binding translation repressor activity
Molecular Function GO:1990825 sequence-specific mRNA binding
Molecular Function GO:0004799 thymidylate synthase activity
Biological Process GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process
Biological Process GO:0071897 DNA biosynthetic process
Biological Process GO:0006260 DNA replication
Biological Process GO:0006231 dTMP biosynthetic process
Biological Process GO:0006235 dTTP biosynthetic process
Biological Process GO:0032259 methylation
Biological Process GO:0017148 negative regulation of translation
Biological Process GO:0035999 tetrahydrofolate interconversion

Reference

[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.