Overview
| Uniprot ID | P04839 |
|---|
| Protein Name | NADPH oxidase 2 |
|---|
| Gene Name | CYBB |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 44 |
PKFFYTRKLLGSALA |
Function
Catalytic subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed:15338276, PubMed:36241643, PubMed:36413210, PubMed:38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (Probable) (PubMed:38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed:19028840, PubMed:38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (By similarity). NADPH oxidase complex assembly is impaired through interaction with NRROS (By similarity)
Protein Sequence
10
MGNWAVNEGL
20
SIFVILVWLG
30
LNVFLFVWYY
40
RVYDIPPKFF
50
YTRKLLGSAL
60
ALARAPAACL
70
NFNCMLILLP
80
VCRNLLSFLR
90
GSSACCSTRV
100
RRQLDRNLTF
110
HKMVAWMIAL
120
HSAIHTIAHL
130
FNVEWCVNAR
140
VNNSDPYSVA
150
LSELGDRQNE
160
SYLNFARKRI
170
KNPEGGLYLA
180
VTLLAGITGV
190
VITLCLILII
200
TSSTKTIRRS
210
YFEVFWYTHH
220
LFVIFFIGLA
230
IHGAERIVRG
240
QTAESLAVHN
250
ITVCEQKISE
260
WGKIKECPIP
270
QFAGNPPMTW
280
KWIVGPMFLY
290
LCERLVRFWR
300
SQQKVVITKV
310
VTHPFKTIEL
320
QMKKKGFKME
330
VGQYIFVKCP
340
KVSKLEWHPF
350
TLTSAPEEDF
360
FSIHIRIVGD
370
WTEGLFNACG
380
CDKQEFQDAW
390
KLPKIAVDGP
400
FGTASEDVFS
410
YEVVMLVGAG
420
IGVTPFASIL
430
KSVWYKYCNN
440
ATNLKLKKIY
450
FYWLCRDTHA
460
FEWFADLLQL
470
LESQMQERNN
480
AGFLSYNIYL
490
TGWDESQANH
500
FAVHHDEEKD
510
VITGLKQKTL
520
YGRPNWDNEF
530
KTIASQHPNT
540
RIGVFLCGPE
550
ALAETLSKQS
560
ISNSESGPRG
570
VHFIFNKENF
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0030425 |
dendrite |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0034702 |
monoatomic ion channel complex |
| Cellular Component |
GO:0043020 |
NADPH oxidase complex |
| Cellular Component |
GO:0043025 |
neuronal cell body |
| Cellular Component |
GO:0005635 |
nuclear envelope |
| Cellular Component |
GO:0097038 |
perinuclear endoplasmic reticulum |
| Cellular Component |
GO:0030670 |
phagocytic vesicle membrane |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0035579 |
specific granule membrane |
| Cellular Component |
GO:0070821 |
tertiary granule membrane |
| Molecular Function |
GO:0071949 |
FAD binding |
| Molecular Function |
GO:0050660 |
flavin adenine dinucleotide binding |
| Molecular Function |
GO:0020037 |
heme binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0016174 |
NAD(P)H oxidase H2O2-forming activity |
| Molecular Function |
GO:0070402 |
NADPH binding |
| Molecular Function |
GO:0046982 |
protein heterodimerization activity |
| Molecular Function |
GO:0016175 |
superoxide-generating NAD(P)H oxidase activity |
| Molecular Function |
GO:0106292 |
superoxide-generating NADPH oxidase activity |
| Biological Process |
GO:0071276 |
cellular response to cadmium ion |
| Biological Process |
GO:0071361 |
cellular response to ethanol |
| Biological Process |
GO:1904845 |
cellular response to L-glutamine |
| Biological Process |
GO:0006952 |
defense response |
| Biological Process |
GO:0097411 |
hypoxia-inducible factor-1alpha signaling pathway |
| Biological Process |
GO:0006954 |
inflammatory response |
| Biological Process |
GO:0045087 |
innate immune response |
| Biological Process |
GO:0034220 |
monoatomic ion transmembrane transport |
| Biological Process |
GO:0045766 |
positive regulation of angiogenesis |
| Biological Process |
GO:0032760 |
positive regulation of tumor necrosis factor production |
| Biological Process |
GO:0045730 |
respiratory burst |
| Biological Process |
GO:1904044 |
response to aldosterone |
| Biological Process |
GO:1990776 |
response to angiotensin |
| Biological Process |
GO:0007584 |
response to nutrient |
| Biological Process |
GO:0009410 |
response to xenobiotic stimulus |
| Biological Process |
GO:0042554 |
superoxide anion generation |
| Biological Process |
GO:0006801 |
superoxide metabolic process |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.