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Overview

Uniprot ID	P04844
Protein Name	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
Gene Name	RPN2
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
392	KDQSIAPKTTRVTYP
442	FVRLHNQKTGQEVVF
627	MLAQQAVKRTAH***
70	AQVPDAKKACTYIRS

Function

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity

Protein Sequence

10 MAPPGSSTVF 20 LLALTIIAST 30 WALTPTHYLT 40 KHDVERLKAS 50 LDRPFTNLES 60 AFYSIVGLSS 70 LGAQVPDAKK 80 ACTYIRSNLD 90 PSNVDSLFYA 100 AQASQALSGC 110 EISISNETKD 120 LLLAAVSEDS 130 SVTQIYHAVA 140 ALSGFGLPLA 150 SQEALSALTA 160 RLSKEETVLA 170 TVQALQTASH 180 LSQQADLRSI 190 VEEIEDLVAR 200 LDELGGVYLQ 210 FEEGLETTAL 220 FVAATYKLMD 230 HVGTEPSIKE 240 DQVIQLMNAI 250 FSKKNFESLS 260 EAFSVASAAA 270 VLSHNRYHVP 280 VVVVPEGSAS 290 DTHEQAILRL 300 QVTNVLSQPL 310 TQATVKLEHA 320 KSVASRATVL 330 QKTSFTPVGD 340 VFELNFMNVK 350 FSSGYYDFLV 360 EVEGDNRYIA 370 NTVELRVKIS 380 TEVGITNVDL 390 STVDKDQSIA 400 PKTTRVTYPA 410 KAKGTFIADS 420 HQNFALFFQL 430 VDVNTGAELT 440 PHQTFVRLHN 450 QKTGQEVVFV 460 AEPDNKNVYK 470 FELDTSERKI 480 EFDSASGTYT 490 LYLIIGDATL 500 KNPILWNVAD 510 VVIKFPEEEA 520 PSTVLSQNLF 530 TPKQEIQHLF 540 REPEKRPPTV 550 VSNTFTALIL 560 SPLLLLFALW 570 IRIGANVSNF 580 TFAPSTIIFH 590 LGHAAMLGLM 600 YVYWTQLNMF 610 QTLKYLAILG 620 SVTFLAGNRM 630 LAQQAVKRTA H

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005783	endoplasmic reticulum
Cellular Component	GO:0005789	endoplasmic reticulum membrane
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0008250	oligosaccharyltransferase complex
Cellular Component	GO:0160226	oligosaccharyltransferase complex A
Cellular Component	GO:0160227	oligosaccharyltransferase complex B
Biological Process	GO:0036211	protein modification process
Biological Process	GO:0006487	protein N-linked glycosylation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.