Overview
| Uniprot ID | P05129 |
|---|
| Protein Name | Protein kinase C gamma type |
|---|
| Gene Name | PRKCG |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 199 |
SDPYVKLKLIPDPRN |
Function
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (By similarity). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock (By similarity). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed:36040231)
Protein Sequence
10
MAGLGPGVGD
20
SEGGPRPLFC
30
RKGALRQKVV
40
HEVKSHKFTA
50
RFFKQPTFCS
60
HCTDFIWGIG
70
KQGLQCQVCS
80
FVVHRRCHEF
90
VTFECPGAGK
100
GPQTDDPRNK
110
HKFRLHSYSS
120
PTFCDHCGSL
130
LYGLVHQGMK
140
CSCCEMNVHR
150
RCVRSVPSLC
160
GVDHTERRGR
170
LQLEIRAPTA
180
DEIHVTVGEA
190
RNLIPMDPNG
200
LSDPYVKLKL
210
IPDPRNLTKQ
220
KTRTVKATLN
230
PVWNETFVFN
240
LKPGDVERRL
250
SVEVWDWDRT
260
SRNDFMGAMS
270
FGVSELLKAP
280
VDGWYKLLNQ
290
EEGEYYNVPV
300
ADADNCSLLQ
310
KFEACNYPLE
320
LYERVRMGPS
330
SSPIPSPSPS
340
PTDPKRCFFG
350
ASPGRLHISD
360
FSFLMVLGKG
370
SFGKVMLAER
380
RGSDELYAIK
390
ILKKDVIVQD
400
DDVDCTLVEK
410
RVLALGGRGP
420
GGRPHFLTQL
430
HSTFQTPDRL
440
YFVMEYVTGG
450
DLMYHIQQLG
460
KFKEPHAAFY
470
AAEIAIGLFF
480
LHNQGIIYRD
490
LKLDNVMLDA
500
EGHIKITDFG
510
MCKENVFPGT
520
TTRTFCGTPD
530
YIAPEIIAYQ
540
PYGKSVDWWS
550
FGVLLYEMLA
560
GQPPFDGEDE
570
EELFQAIMEQ
580
TVTYPKSLSR
590
EAVAICKGFL
600
TKHPGKRLGS
610
GPDGEPTIRA
620
HGFFRWIDWE
630
RLERLEIPPP
640
FRPRPCGRSG
650
ENFDKFFTRA
660
APALTPPDRL
670
VLASIDQADF
680
QGFTYVNPDF
690
VHPDARSPTS
PVPVPVM
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0044305 |
calyx of Held |
| Cellular Component |
GO:0005911 |
cell-cell junction |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0030425 |
dendrite |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0099524 |
postsynaptic cytosol |
| Cellular Component |
GO:0014069 |
postsynaptic density |
| Cellular Component |
GO:0099523 |
presynaptic cytosol |
| Cellular Component |
GO:0097060 |
synaptic membrane |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0004698 |
calcium,diacylglycerol-dependent serine/threonine kinase activity |
| Molecular Function |
GO:0004697 |
diacylglycerol-dependent serine/threonine kinase activity |
| Molecular Function |
GO:0004672 |
protein kinase activity |
| Molecular Function |
GO:0106310 |
protein serine kinase activity |
| Molecular Function |
GO:0004674 |
protein serine/threonine kinase activity |
| Molecular Function |
GO:0004712 |
protein serine/threonine/tyrosine kinase activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0007268 |
chemical synaptic transmission |
| Biological Process |
GO:0060384 |
innervation |
| Biological Process |
GO:0035556 |
intracellular signal transduction |
| Biological Process |
GO:0007611 |
learning or memory |
| Biological Process |
GO:0060291 |
long-term synaptic potentiation |
| Biological Process |
GO:0043524 |
negative regulation of neuron apoptotic process |
| Biological Process |
GO:1901799 |
negative regulation of proteasomal protein catabolic process |
| Biological Process |
GO:0042177 |
negative regulation of protein catabolic process |
| Biological Process |
GO:0031397 |
negative regulation of protein ubiquitination |
| Biological Process |
GO:0007200 |
phospholipase C-activating G protein-coupled receptor signaling pathway |
| Biological Process |
GO:0032425 |
positive regulation of mismatch repair |
| Biological Process |
GO:0042752 |
regulation of circadian rhythm |
| Biological Process |
GO:0032095 |
regulation of response to food |
| Biological Process |
GO:2000300 |
regulation of synaptic vesicle exocytosis |
| Biological Process |
GO:1990776 |
response to angiotensin |
| Biological Process |
GO:0043278 |
response to morphine |
| Biological Process |
GO:0048265 |
response to pain |
| Biological Process |
GO:1990911 |
response to psychosocial stress |
| Biological Process |
GO:0009636 |
response to toxic substance |
| Biological Process |
GO:0048511 |
rhythmic process |
Reference
[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.