Search Results

Overview

Uniprot IDP05166
Protein NamePropionyl-CoA carboxylase beta chain, mitochondrial
Gene NamePCCB
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
248 QEELGGAKTHTTMSG
373 LDINSSVKGARFVRF
433 KVTVITRKAYGGAYD
474 GAVEIIFKGHENVEA
525 DLDVLASKKVQRPWR
60 RRIDAQHKRGKLTAR
99 DFGMAADKNKFPGDS

Function

This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed:15890657, PubMed:6765947). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (PubMed:15890657, PubMed:6765947). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate (PubMed:6765947). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity)

Protein Sequence

10 MAAALRVAAV 20 GARLSVLASG 30 LRAAVRSLCS 40 QATSVNERIE 50 NKRRTALLGG 60 GQRRIDAQHK 70 RGKLTARERI 80 SLLLDPGSFV 90 ESDMFVEHRC 100 ADFGMAADKN 110 KFPGDSVVTG 120 RGRINGRLVY 130 VFSQDFTVFG 140 GSLSGAHAQK 150 ICKIMDQAIT 160 VGAPVIGLND 170 SGGARIQEGV 180 ESLAGYADIF 190 LRNVTASGVI 200 PQISLIMGPC 210 AGGAVYSPAL 220 TDFTFMVKDT 230 SYLFITGPDV 240 VKSVTNEDVT 250 QEELGGAKTH 260 TTMSGVAHRA 270 FENDVDALCN 280 LRDFFNYLPL 290 SSQDPAPVRE 300 CHDPSDRLVP 310 ELDTIVPLES 320 TKAYNMVDII 330 HSVVDEREFF 340 EIMPNYAKNI 350 IVGFARMNGR 360 TVGIVGNQPK 370 VASGCLDINS 380 SVKGARFVRF 390 CDAFNIPLIT 400 FVDVPGFLPG 410 TAQEYGGIIR 420 HGAKLLYAFA 430 EATVPKVTVI 440 TRKAYGGAYD 450 VMSSKHLCGD 460 TNYAWPTAEI 470 AVMGAKGAVE 480 IIFKGHENVE 490 AAQAEYIEKF 500 ANPFPAAVRG 510 FVDDIIQPSS 520 TRARICCDLD 530 VLASKKVQRP WRKHANIPL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004658 propionyl-CoA carboxylase activity
Biological Process GO:0009081 branched-chain amino acid metabolic process
Biological Process GO:0006631 fatty acid metabolic process
Biological Process GO:0019626 short-chain fatty acid catabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.