Overview
| Uniprot ID | P05177 |
|---|
| Protein Name | Cytochrome P450 1A2 |
|---|
| Gene Name | CYP1A2 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 254 |
NPALQRFKAFNQRFL |
| 277 |
EHYQDFDKNSVRDIT |
| 289 |
DITGALFKHSKKGPR |
| 442 |
ADGTAINKPLSEKMM |
Function
A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576, PubMed:9435160). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576, PubMed:9435160). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed:11555828, PubMed:12865317). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2 (PubMed:11555828, PubMed:12865317). Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed:21576599). May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376). Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer (PubMed:19965576). Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA (PubMed:9435160). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed:21068195). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin (PubMed:14725854). Metabolizes caffeine via N3-demethylation (Probable)
Protein Sequence
10
MALSQSVPFS
20
ATELLLASAI
30
FCLVFWVLKG
40
LRPRVPKGLK
50
SPPEPWGWPL
60
LGHVLTLGKN
70
PHLALSRMSQ
80
RYGDVLQIRI
90
GSTPVLVLSR
100
LDTIRQALVR
110
QGDDFKGRPD
120
LYTSTLITDG
130
QSLTFSTDSG
140
PVWAARRRLA
150
QNALNTFSIA
160
SDPASSSSCY
170
LEEHVSKEAK
180
ALISRLQELM
190
AGPGHFDPYN
200
QVVVSVANVI
210
GAMCFGQHFP
220
ESSDEMLSLV
230
KNTHEFVETA
240
SSGNPLDFFP
250
ILRYLPNPAL
260
QRFKAFNQRF
270
LWFLQKTVQE
280
HYQDFDKNSV
290
RDITGALFKH
300
SKKGPRASGN
310
LIPQEKIVNL
320
VNDIFGAGFD
330
TVTTAISWSL
340
MYLVTKPEIQ
350
RKIQKELDTV
360
IGRERRPRLS
370
DRPQLPYLEA
380
FILETFRHSS
390
FLPFTIPHST
400
TRDTTLNGFY
410
IPKKCCVFVN
420
QWQVNHDPEL
430
WEDPSEFRPE
440
RFLTADGTAI
450
NKPLSEKMML
460
FGMGKRRCIG
470
EVLAKWEIFL
480
FLAILLQQLE
490
FSVPPGVKVD
500
LTPIYGLTMK
510
HARCEHVQAR
LRFSIN
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0043231 |
intracellular membrane-bounded organelle |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Molecular Function |
GO:0034875 |
caffeine oxidase activity |
| Molecular Function |
GO:0032451 |
demethylase activity |
| Molecular Function |
GO:0009055 |
electron transfer activity |
| Molecular Function |
GO:0019899 |
enzyme binding |
| Molecular Function |
GO:0101020 |
estrogen 16-alpha-hydroxylase activity |
| Molecular Function |
GO:0101021 |
estrogen 2-hydroxylase activity |
| Molecular Function |
GO:0020037 |
heme binding |
| Molecular Function |
GO:0106256 |
hydroperoxy icosatetraenoate dehydratase activity |
| Molecular Function |
GO:0005506 |
iron ion binding |
| Molecular Function |
GO:0004497 |
monooxygenase activity |
| Molecular Function |
GO:0016491 |
oxidoreductase activity |
| Molecular Function |
GO:0016712 |
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen |
| Molecular Function |
GO:0008395 |
steroid hydroxylase activity |
| Biological Process |
GO:0046222 |
aflatoxin metabolic process |
| Biological Process |
GO:0009820 |
alkaloid metabolic process |
| Biological Process |
GO:0008203 |
cholesterol metabolic process |
| Biological Process |
GO:0019373 |
epoxygenase P450 pathway |
| Biological Process |
GO:0008210 |
estrogen metabolic process |
| Biological Process |
GO:0042759 |
long-chain fatty acid biosynthetic process |
| Biological Process |
GO:0032259 |
methylation |
| Biological Process |
GO:0032787 |
monocarboxylic acid metabolic process |
| Biological Process |
GO:0016098 |
monoterpenoid metabolic process |
| Biological Process |
GO:0097267 |
omega-hydroxylase P450 pathway |
| Biological Process |
GO:0070989 |
oxidative demethylation |
| Biological Process |
GO:0042572 |
retinol metabolic process |
| Biological Process |
GO:0006706 |
steroid catabolic process |
| Biological Process |
GO:0009403 |
toxin biosynthetic process |
| Biological Process |
GO:0009404 |
toxin metabolic process |
| Biological Process |
GO:0042178 |
xenobiotic catabolic process |
| Biological Process |
GO:0006805 |
xenobiotic metabolic process |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.