Overview
| Uniprot ID | P05181 |
| Protein Name | Cytochrome P450 2E1 |
| Gene Name | CYP2E1 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 140 |
LRNYGMGKQGNESRI |
| 187 |
IADILFRKHFDYNDE |
| 243 |
IKNVAEVKEYVSERV |
| 84 |
MVVMHGYKAVKEALL |
| 87 |
MHGYKAVKEALLDYK |
| 94 |
KEALLDYKDEFSGRG |
Function
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed:10553002, PubMed:18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:10553002, PubMed:18577768). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids (PubMed:10553002, PubMed:18577768). May be involved in the oxidative metabolism of xenobiotics (Probable)
Protein Sequence
10
MSALGVTVAL
20
LVWAAFLLLV
30
SMWRQVHSSW
40
NLPPGPFPLP
50
IIGNLFQLEL
60
KNIPKSFTRL
70
AQRFGPVFTL
80
YVGSQRMVVM
90
HGYKAVKEAL
100
LDYKDEFSGR
110
GDLPAFHAHR
120
DRGIIFNNGP
130
TWKDIRRFSL
140
TTLRNYGMGK
150
QGNESRIQRE
160
AHFLLEALRK
170
TQGQPFDPTF
180
LIGCAPCNVI
190
ADILFRKHFD
200
YNDEKFLRLM
210
YLFNENFHLL
220
STPWLQLYNN
230
FPSFLHYLPG
240
SHRKVIKNVA
250
EVKEYVSERV
260
KEHHQSLDPN
270
CPRDLTDCLL
280
VEMEKEKHSA
290
ERLYTMDGIT
300
VTVADLFFAG
310
TETTSTTLRY
320
GLLILMKYPE
330
IEEKLHEEID
340
RVIGPSRIPA
350
IKDRQEMPYM
360
DAVVHEIQRF
370
ITLVPSNLPH
380
EATRDTIFRG
390
YLIPKGTVVV
400
PTLDSVLYDN
410
QEFPDPEKFK
420
PEHFLNENGK
430
FKYSDYFKPF
440
STGKRVCAGE
450
GLARMELFLL
460
LCAILQHFNL
470
KPLVDPKDID
480
LSPIHIGFGC
490
IPPRYKLCVI
PRS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Biological Process |
GO:0001676 |
long-chain fatty acid metabolic process |
| Biological Process |
GO:0016098 |
monoterpenoid metabolic process |
| Biological Process |
GO:0008202 |
steroid metabolic process |
| Biological Process |
GO:0006805 |
xenobiotic metabolic process |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0005743 |
mitochondrial inner membrane |
| Molecular Function |
GO:0018601 |
4-nitrophenol 2-monooxygenase activity |
| Molecular Function |
GO:0008392 |
arachidonate epoxygenase activity |
| Molecular Function |
GO:0019899 |
enzyme binding |
| Molecular Function |
GO:0020037 |
heme binding |
| Molecular Function |
GO:0030544 |
Hsp70 protein binding |
| Molecular Function |
GO:0051879 |
Hsp90 protein binding |
| Molecular Function |
GO:0005506 |
iron ion binding |
| Molecular Function |
GO:0120319 |
long-chain fatty acid omega-1 hydroxylase activity |
| Molecular Function |
GO:0004497 |
monooxygenase activity |
| Molecular Function |
GO:0016491 |
oxidoreductase activity |
| Molecular Function |
GO:0016709 |
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen |
| Molecular Function |
GO:0016712 |
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen |
| Molecular Function |
GO:0019825 |
oxygen binding |
| Biological Process |
GO:0018960 |
4-nitrophenol metabolic process |
| Biological Process |
GO:0018910 |
benzene metabolic process |
| Biological Process |
GO:0018885 |
carbon tetrachloride metabolic process |
| Biological Process |
GO:0019373 |
epoxygenase P450 pathway |
| Biological Process |
GO:0042197 |
halogenated hydrocarbon metabolic process |
| Biological Process |
GO:0002933 |
lipid hydroxylation |
| Biological Process |
GO:0042759 |
long-chain fatty acid biosynthetic process |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.