Search Results

Overview

Uniprot IDP05708
Protein NameHexokinase-1
Gene NameHk1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
176 ITWTKRFKASGVEGA
187 VEGADVVKLLNKAIK
24 DQVKKIDKYLYAMRL
290 RGSLNPGKQLFEKMV
315 LILVKMAKEGLLFEG
333 PELLTRGKFNTSDVS
344 SDVSAIEKDKEGIQN
346 VSAIEKDKEGIQNAK
418 GVDGSLYKMHPQYSR
451 LSESGTGKGAAMVTA
481 LAHFRLSKQTLMEVK
506 LRKETNSKATVKMLP
510 TNSKATVKMLPSFVR
727 DIRTDFDKVVDEYSL
738 EYSLNSGKQRFEKMI
763 NILIDFTKKGFLFRG
777 GQISEPLKTRGIFET
828 TVCGVVSKRAAQLCG
866 GVDGTLYKLHPHFSR
880 RIMHQTVKELSPKCT

Function

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:13211595, PubMed:3579310, PubMed:5871820). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:13211595, PubMed:5871820). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (By similarity). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (By similarity)

Protein Sequence

10 MIAAQLLAYY 20 FTELKDDQVK 30 KIDKYLYAMR 40 LSDEILIDIL 50 TRFKKEMKNG 60 LSRDYNPTAS 70 VKMLPTFVRS 80 IPDGSEKGDF 90 IALDLGGSSF 100 RILRVQVNHE 110 KNQNVSMESE 120 IYDTPENIVH 130 GSGTQLFDHV 140 ADCLGDFMEK 150 KKIKDKKLPV 160 GFTFSFPCRQ 170 SKIDEAVLIT 180 WTKRFKASGV 190 EGADVVKLLN 200 KAIKKRGDYD 210 ANIVAVVNDT 220 VGTMMTCGYD 230 DQQCEVGLII 240 GTGTNACYME 250 ELRHIDLVEG 260 DEGRMCINTE 270 WGAFGDDGSL 280 EDIRTEFDRE 290 LDRGSLNPGK 300 QLFEKMVSGM 310 YMGELVRLIL 320 VKMAKEGLLF 330 EGRITPELLT 340 RGKFNTSDVS 350 AIEKDKEGIQ 360 NAKEILTRLG 370 VEPSDVDCVS 380 VQHICTIVSF 390 RSANLVAATL 400 GAILNRLRDN 410 KGTPRLRTTV 420 GVDGSLYKMH 430 PQYSRRFHKT 440 LRRLVPDSDV 450 RFLLSESGTG 460 KGAAMVTAVA 470 YRLAEQHRQI 480 EETLAHFRLS 490 KQTLMEVKKR 500 LRTEMEMGLR 510 KETNSKATVK 520 MLPSFVRSIP 530 DGTEHGDFLA 540 LDLGGTNFRV 550 LLVKIRSGKK 560 RTVEMHNKIY 570 SIPLEIMQGT 580 GDELFDHIVS 590 CISDFLDYMG 600 IKGPRMPLGF 610 TFSFPCHQTN 620 LDCGILISWT 630 KGFKATDCEG 640 HDVASLLRDA 650 VKRREEFDLD 660 VVAVVNDTVG 670 TMMTCAYEEP 680 TCEIGLIVGT 690 GTNACYMEEM 700 KNVEMVEGNQ 710 GQMCINMEWG 720 AFGDNGCLDD 730 IRTDFDKVVD 740 EYSLNSGKQR 750 FEKMISGMYL 760 GEIVRNILID 770 FTKKGFLFRG 780 QISEPLKTRG 790 IFETKFLSQI 800 ESDRLALLQV 810 RAILQQLGLN 820 STCDDSILVK 830 TVCGVVSKRA 840 AQLCGAGMAA 850 VVEKIRENRG 860 LDHLNVTVGV 870 DGTLYKLHPH 880 FSRIMHQTVK 890 ELSPKCTVSF 900 LLSEDGSGKG 910 AALITAVGVR LRGDPSIA

Gene Ontology

Classification GO ID Description
Biological Process GO:0006954 inflammatory response
Cellular Component GO:0005901 caveola
Cellular Component GO:0005929 cilium
Cellular Component GO:0005829 cytosol
Cellular Component GO:0045121 membrane raft
Cellular Component GO:0005741 mitochondrial outer membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0097228 sperm principal piece
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0005536 D-glucose binding
Molecular Function GO:0008865 fructokinase activity
Molecular Function GO:0004340 glucokinase activity
Molecular Function GO:0047931 glucosamine kinase activity
Molecular Function GO:0004396 hexokinase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0019158 mannokinase activity
Molecular Function GO:0042834 peptidoglycan binding
Molecular Function GO:0004672 protein kinase activity
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0046835 carbohydrate phosphorylation
Biological Process GO:0072655 establishment of protein localization to mitochondrion
Biological Process GO:0006002 fructose 6-phosphate metabolic process
Biological Process GO:0051156 glucose 6-phosphate metabolic process
Biological Process GO:0006006 glucose metabolic process
Biological Process GO:0006096 glycolytic process
Biological Process GO:0045087 innate immune response
Biological Process GO:0001678 intracellular glucose homeostasis
Biological Process GO:0072656 maintenance of protein location in mitochondrion
Biological Process GO:0006013 mannose metabolic process
Biological Process GO:1903960 negative regulation of anion transmembrane transport
Biological Process GO:0043066 negative regulation of apoptotic process
Biological Process GO:0018105 peptidyl-serine phosphorylation
Biological Process GO:0018107 peptidyl-threonine phosphorylation
Biological Process GO:0018108 peptidyl-tyrosine phosphorylation
Biological Process GO:0002720 positive regulation of cytokine production involved in immune response
Biological Process GO:0032731 positive regulation of interleukin-1 beta production
Biological Process GO:0046777 protein autophosphorylation
Biological Process GO:0006468 protein phosphorylation
Biological Process GO:0009741 response to brassinosteroid
Biological Process GO:0001666 response to hypoxia
Biological Process GO:0002931 response to ischemia
Biological Process GO:1901986 response to ketamine

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.