Search Results

Overview

Uniprot IDP05982
Protein NameNAD(P)H dehydrogenase [quinone] 1
Gene NameNqo1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
209 VQVLEGWKKRLETVW
251 QEEQKKNKFGLSVGH
31 EAAVEALKKKGWEVV

Function

Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species (PubMed:1703398, PubMed:7862630, PubMed:8999809). Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion (By similarity). Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential (By similarity). Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome (By similarity)

Protein Sequence

10 MAVRRALIVL 20 AHAERTSFNY 30 AMKEAAVEAL 40 KKKGWEVVES 50 DLYAMNFNPL 60 ISRNDITGEP 70 KDSENFQYPV 80 ESSLAYKEGR 90 LSPDIVAEQK 100 KLEAADLVIF 110 QFPLYWFGVP 120 AILKGWFERV 130 LVAGFAYTYA 140 TMYDKGPFQN 150 KKTLLSITTG 160 GSGSMYSLQG 170 VHGDMNVILW 180 PIQSGILRFC 190 GFQVLEPQLV 200 YSIGHTPPDA 210 RVQVLEGWKK 220 RLETVWEESP 230 LYFAPSSLFD 240 LNFQAGFLLK 250 KEVQEEQKKN 260 KFGLSVGHHL 270 GKSIPADNQI KARK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0030425 dendrite
Cellular Component GO:0043025 neuronal cell body
Cellular Component GO:0005634 nucleus
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0003955 NAD(P)H dehydrogenase (quinone) activity
Molecular Function GO:0050136 NADH dehydrogenase (quinone) (non-electrogenic) activity
Molecular Function GO:0008753 NADPH dehydrogenase (quinone) activity
Molecular Function GO:0004784 superoxide dismutase activity
Biological Process GO:0045454 cell redox homeostasis
Biological Process GO:0070301 cellular response to hydrogen peroxide
Biological Process GO:0071248 cellular response to metal ion
Biological Process GO:0034599 cellular response to oxidative stress
Biological Process GO:0045087 innate immune response
Biological Process GO:0043066 negative regulation of apoptotic process
Biological Process GO:0043086 negative regulation of catalytic activity
Biological Process GO:0110076 negative regulation of ferroptosis
Biological Process GO:0042177 negative regulation of protein catabolic process
Biological Process GO:1902690 positive regulation of NAD metabolic process
Biological Process GO:0043525 positive regulation of neuron apoptotic process
Biological Process GO:0030163 protein catabolic process
Biological Process GO:0000209 protein polyubiquitination
Biological Process GO:0019430 removal of superoxide radicals
Biological Process GO:0043279 response to alkaloid
Biological Process GO:0061771 response to caloric restriction
Biological Process GO:0009743 response to carbohydrate
Biological Process GO:0051602 response to electrical stimulus
Biological Process GO:0032355 response to estradiol
Biological Process GO:0045471 response to ethanol
Biological Process GO:1905395 response to flavonoid
Biological Process GO:0009725 response to hormone
Biological Process GO:1904880 response to hydrogen sulfide
Biological Process GO:0002931 response to ischemia
Biological Process GO:1904844 response to L-glutamine
Biological Process GO:0032496 response to lipopolysaccharide
Biological Process GO:0007584 response to nutrient
Biological Process GO:0006979 response to oxidative stress
Biological Process GO:0033574 response to testosterone
Biological Process GO:1904772 response to tetrachloromethane
Biological Process GO:0009410 response to xenobiotic stimulus
Biological Process GO:0006801 superoxide metabolic process
Biological Process GO:0006743 ubiquinone metabolic process
Biological Process GO:0042360 vitamin E metabolic process
Biological Process GO:0042373 vitamin K metabolic process

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.