Search Results

Overview

Uniprot IDP06400
Protein NameRetinoblastoma-associated protein
Gene NameRB1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
265 NRSARIAKQLENDTR
791 HIPRSPYKFPSSPLR
824 EGLPTPTKMTPRSRI
847 GTSEKFQKINQMVCN
873 SNPPKPLKKLRFDIE
874 NPPKPLKKLRFDIEG
900 GESKFQQKLAEMTST

Function

Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity)

Protein Sequence

10 MPPKTPRKTA 20 ATAAAAAAEP 30 PAPPPPPPPE 40 EDPEQDSGPE 50 DLPLVRLEFE 60 ETEEPDFTAL 70 CQKLKIPDHV 80 RERAWLTWEK 90 VSSVDGVLGG 100 YIQKKKELWG 110 ICIFIAAVDL 120 DEMSFTFTEL 130 QKNIEISVHK 140 FFNLLKEIDT 150 STKVDNAMSR 160 LLKKYDVLFA 170 LFSKLERTCE 180 LIYLTQPSSS 190 ISTEINSALV 200 LKVSWITFLL 210 AKGEVLQMED 220 DLVISFQLML 230 CVLDYFIKLS 240 PPMLLKEPYK 250 TAVIPINGSP 260 RTPRRGQNRS 270 ARIAKQLEND 280 TRIIEVLCKE 290 HECNIDEVKN 300 VYFKNFIPFM 310 NSLGLVTSNG 320 LPEVENLSKR 330 YEEIYLKNKD 340 LDARLFLDHD 350 KTLQTDSIDS 360 FETQRTPRKS 370 NLDEEVNVIP 380 PHTPVRTVMN 390 TIQQLMMILN 400 SASDQPSENL 410 ISYFNNCTVN 420 PKESILKRVK 430 DIGYIFKEKF 440 AKAVGQGCVE 450 IGSQRYKLGV 460 RLYYRVMESM 470 LKSEEERLSI 480 QNFSKLLNDN 490 IFHMSLLACA 500 LEVVMATYSR 510 STSQNLDSGT 520 DLSFPWILNV 530 LNLKAFDFYK 540 VIESFIKAEG 550 NLTREMIKHL 560 ERCEHRIMES 570 LAWLSDSPLF 580 DLIKQSKDRE 590 GPTDHLESAC 600 PLNLPLQNNH 610 TAADMYLSPV 620 RSPKKKGSTT 630 RVNSTANAET 640 QATSAFQTQK 650 PLKSTSLSLF 660 YKKVYRLAYL 670 RLNTLCERLL 680 SEHPELEHII 690 WTLFQHTLQN 700 EYELMRDRHL 710 DQIMMCSMYG 720 ICKVKNIDLK 730 FKIIVTAYKD 740 LPHAVQETFK 750 RVLIKEEEYD 760 SIIVFYNSVF 770 MQRLKTNILQ 780 YASTRPPTLS 790 PIPHIPRSPY 800 KFPSSPLRIP 810 GGNIYISPLK 820 SPYKISEGLP 830 TPTKMTPRSR 840 ILVSIGESFG 850 TSEKFQKINQ 860 MVCNSDRVLK 870 RSAEGSNPPK 880 PLKKLRFDIE 890 GSDEADGSKH 900 LPGESKFQQK 910 LAEMTSTRTR 920 MQKQKMNDSM DTSNKEEK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Biological Process GO:0048667 cell morphogenesis involved in neuron differentiation
Biological Process GO:0032869 cellular response to insulin stimulus
Biological Process GO:0002062 chondrocyte differentiation
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0051276 chromosome organization
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0031507 heterochromatin formation
Biological Process GO:0034088 maintenance of mitotic sister chromatid cohesion
Biological Process GO:0045445 myoblast differentiation
Biological Process GO:2001234 negative regulation of apoptotic signaling pathway
Biological Process GO:0045786 negative regulation of cell cycle
Biological Process GO:0030308 negative regulation of cell growth
Biological Process GO:0120163 negative regulation of cold-induced thermogenesis
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:2000134 negative regulation of G1/S transition of mitotic cell cycle
Biological Process GO:0010629 negative regulation of gene expression
Biological Process GO:1903944 negative regulation of hepatocyte apoptotic process
Biological Process GO:0050728 negative regulation of inflammatory response
Biological Process GO:1904761 negative regulation of myofibroblast differentiation
Biological Process GO:0006469 negative regulation of protein kinase activity
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0031175 neuron projection development
Biological Process GO:1904028 positive regulation of collagen fibril organization
Biological Process GO:1903055 positive regulation of extracellular matrix organization
Biological Process GO:0045842 positive regulation of mitotic metaphase/anaphase transition
Biological Process GO:2000679 positive regulation of transcription regulatory region DNA binding
Biological Process GO:0071459 protein localization to chromosome, centromeric region
Biological Process GO:0007265 Ras protein signal transduction
Biological Process GO:0051726 regulation of cell cycle
Biological Process GO:0006355 regulation of DNA-templated transcription
Biological Process GO:0043550 regulation of lipid kinase activity
Biological Process GO:0007346 regulation of mitotic cell cycle
Biological Process GO:0031134 sister chromatid biorientation
Biological Process GO:0007283 spermatogenesis
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0016605 PML body
Cellular Component GO:0035189 Rb-E2F complex
Cellular Component GO:0005819 spindle
Cellular Component GO:0016514 SWI/SNF complex
Molecular Function GO:0097718 disordered domain specific binding
Molecular Function GO:0140297 DNA-binding transcription factor binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0061676 importin-alpha family protein binding
Molecular Function GO:0019900 kinase binding
Molecular Function GO:0060090 molecular adaptor activity
Molecular Function GO:0051219 phosphoprotein binding
Molecular Function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
Molecular Function GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
Molecular Function GO:0003714 transcription corepressor activity
Molecular Function GO:0031625 ubiquitin protein ligase binding
Biological Process GO:0003180 aortic valve morphogenesis
Biological Process GO:0006915 apoptotic process
Biological Process GO:0030154 cell differentiation

Reference

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[2] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.