Search Results
Overview
| Uniprot ID | P06761 |
|---|---|
| Protein Name | Endoplasmic reticulum chaperone BiP |
| Gene Name | Hspa5 |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 123 | PFKVVEKKTKPYIQV |
| 125 | KVVEKKTKPYIQVDI |
| 154 | AMVLTKMKETAEAYL |
| 163 | TAEAYLGKKVTHAVV |
| 164 | AEAYLGKKVTHAVVT |
| 185 | DAQRQATKDAGTIAG |
| 213 | AIAYGLDKREGEKNI |
| 268 | RVMEHFIKLYKKKTG |
| 340 | DLFRSTMKPVQKVLE |
| 344 | STMKPVQKVLEDSDL |
| 352 | VLEDSDLKKSDIDEI |
| 353 | LEDSDLKKSDIDEIV |
| 370 | GGSTRIPKIQQLVKE |
| 446 | RNTVVPTKKSQIFST |
| 447 | NTVVPTKKSQIFSTA |
| 523 | KGTGNKNKITITNDQ |
| 547 | RMVNDAEKFAEEDKK |
| 553 | EKFAEEDKKLKERID |
| 573 | ESYAYSLKNQIGDKE |
| 81 | RLIGDAAKNQLTSNP |
| 96 | ENTVFDAKRLIGRTW |
Function
Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (By similarity). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (By similarity). Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Also binds and inactivates EIF2AK3/PERK in unstressed cells. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1 and EIF2AK3/PERK, allowing their homodimerization and subsequent activation (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation (By similarity)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0009986 | cell surface |
| Biological Process | GO:0051603 | proteolysis involved in protein catabolic process |
| Biological Process | GO:1903894 | regulation of IRE1-mediated unfolded protein response |
| Biological Process | GO:0042220 | response to cocaine |
| Biological Process | GO:0034976 | response to endoplasmic reticulum stress |
| Biological Process | GO:1904313 | response to methamphetamine hydrochloride |
| Biological Process | GO:0097501 | stress response to metal ion |
| Cellular Component | GO:0008180 | COP9 signalosome |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0043198 | dendritic shaft |
| Cellular Component | GO:0005783 | endoplasmic reticulum |
| Cellular Component | GO:0034663 | endoplasmic reticulum chaperone complex |
| Cellular Component | GO:0005788 | endoplasmic reticulum lumen |
| Cellular Component | GO:0005789 | endoplasmic reticulum membrane |
| Cellular Component | GO:0005793 | endoplasmic reticulum-Golgi intermediate compartment |
| Cellular Component | GO:0042470 | melanosome |
| Cellular Component | GO:0016020 | membrane |
| Cellular Component | GO:0030496 | midbody |
| Cellular Component | GO:0005739 | mitochondrion |
| Cellular Component | GO:0043025 | neuronal cell body |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0005886 | plasma membrane |
| Cellular Component | GO:0032991 | protein-containing complex |
| Cellular Component | GO:0005790 | smooth endoplasmic reticulum |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0016887 | ATP hydrolysis activity |
| Molecular Function | GO:0019899 | enzyme binding |
| Molecular Function | GO:0031072 | heat shock protein binding |
| Molecular Function | GO:0051787 | misfolded protein binding |
| Molecular Function | GO:0019904 | protein domain specific binding |
| Molecular Function | GO:0044183 | protein folding chaperone |
| Molecular Function | GO:0140311 | protein sequestering activity |
| Molecular Function | GO:0030291 | protein serine/threonine kinase inhibitor activity |
| Molecular Function | GO:0043022 | ribosome binding |
| Molecular Function | GO:0031625 | ubiquitin protein ligase binding |
| Molecular Function | GO:0051082 | unfolded protein binding |
| Biological Process | GO:0071236 | cellular response to antibiotic |
| Biological Process | GO:0071277 | cellular response to calcium ion |
| Biological Process | GO:0071320 | cellular response to cAMP |
| Biological Process | GO:0071480 | cellular response to gamma radiation |
| Biological Process | GO:0042149 | cellular response to glucose starvation |
| Biological Process | GO:0071353 | cellular response to interleukin-4 |
| Biological Process | GO:0071287 | cellular response to manganese ion |
| Biological Process | GO:1990090 | cellular response to nerve growth factor stimulus |
| Biological Process | GO:0071466 | cellular response to xenobiotic stimulus |
| Biological Process | GO:0021680 | cerebellar Purkinje cell layer development |
| Biological Process | GO:0021589 | cerebellum structural organization |
| Biological Process | GO:0030968 | endoplasmic reticulum unfolded protein response |
| Biological Process | GO:0006983 | ER overload response |
| Biological Process | GO:0036503 | ERAD pathway |
| Biological Process | GO:0036498 | IRE1-mediated unfolded protein response |
| Biological Process | GO:0001554 | luteolysis |
| Biological Process | GO:0035437 | maintenance of protein localization in endoplasmic reticulum |
| Biological Process | GO:0043066 | negative regulation of apoptotic process |
| Biological Process | GO:1903895 | negative regulation of IRE1-mediated unfolded protein response |
| Biological Process | GO:1903898 | negative regulation of PERK-mediated unfolded protein response |
| Biological Process | GO:0031333 | negative regulation of protein-containing complex assembly |
| Biological Process | GO:0030512 | negative regulation of transforming growth factor beta receptor signaling pathway |
| Biological Process | GO:0051402 | neuron apoptotic process |
| Biological Process | GO:0030182 | neuron differentiation |
| Biological Process | GO:0030335 | positive regulation of cell migration |
| Biological Process | GO:0010976 | positive regulation of neuron projection development |
| Biological Process | GO:0031398 | positive regulation of protein ubiquitination |
| Biological Process | GO:0031204 | post-translational protein targeting to membrane, translocation |
| Biological Process | GO:0042026 | protein refolding |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.
[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.