Overview
| Uniprot ID | P06766 |
|---|
| Protein Name | DNA polymerase beta |
|---|
| Gene Name | Polb |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 48 |
KAASVIAKYPHKIKS |
Function
Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase
Protein Sequence
10
MSKRKAPQET
20
LNGGITDMLV
30
ELANFEKNVS
40
QAIHKYNAYR
50
KAASVIAKYP
60
HKIKSGAEAK
70
KLPGVGTKIA
80
EKIDEFLATG
90
KLRKLEKIRQ
100
DDTSSSINFL
110
TRVTGIGPSA
120
ARKLVDEGIK
130
TLEDLRKNED
140
KLNHHQRIGL
150
KYFEDFEKRI
160
PREEMLQMQD
170
IVLNEVKKLD
180
PEYIATVCGS
190
FRRGAESSGD
200
MDVLLTHPNF
210
TSESSKQPKL
220
LHRVVEQLQK
230
VRFITDTLSK
240
GETKFMGVCQ
250
LPSENDENEY
260
PHRRIDIRLI
270
PKDQYYCGVL
280
YFTGSDIFNK
290
NMRAHALEKG
300
FTINEYTIRP
310
LGVTGVAGEP
320
LPVDSEQDIF
330
DYIQWRYREP
KDRSE
Gene Ontology
| Classification |
GO ID |
Description |
| Molecular Function |
GO:0019899 |
enzyme binding |
| Molecular Function |
GO:0016829 |
lyase activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0008017 |
microtubule binding |
| Biological Process |
GO:0006915 |
apoptotic process |
| Biological Process |
GO:0006284 |
base-excision repair |
| Biological Process |
GO:0006287 |
base-excision repair, gap-filling |
| Biological Process |
GO:0006974 |
DNA damage response |
| Biological Process |
GO:0006260 |
DNA replication |
| Biological Process |
GO:0006303 |
double-strand break repair via nonhomologous end joining |
| Biological Process |
GO:0048872 |
homeostasis of number of cells |
| Biological Process |
GO:0071707 |
immunoglobulin heavy chain V-D-J recombination |
| Biological Process |
GO:0001701 |
in utero embryonic development |
| Biological Process |
GO:0006954 |
inflammatory response |
| Biological Process |
GO:0008630 |
intrinsic apoptotic signaling pathway in response to DNA damage |
| Biological Process |
GO:0048535 |
lymph node development |
| Biological Process |
GO:0051402 |
neuron apoptotic process |
| Biological Process |
GO:0006290 |
pyrimidine dimer repair |
| Biological Process |
GO:0045471 |
response to ethanol |
| Biological Process |
GO:0010332 |
response to gamma radiation |
| Biological Process |
GO:0055093 |
response to hyperoxia |
| Biological Process |
GO:0007435 |
salivary gland morphogenesis |
| Biological Process |
GO:0016445 |
somatic diversification of immunoglobulins |
| Biological Process |
GO:0016446 |
somatic hypermutation of immunoglobulin genes |
| Biological Process |
GO:0048536 |
spleen development |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005874 |
microtubule |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Cellular Component |
GO:0005876 |
spindle microtubule |
| Molecular Function |
GO:0051575 |
5'-deoxyribose-5-phosphate lyase activity |
| Molecular Function |
GO:0140078 |
class I DNA-(apurinic or apyrimidinic site) endonuclease activity |
| Molecular Function |
GO:0003684 |
damaged DNA binding |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0003887 |
DNA-directed DNA polymerase activity |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.