Search Results

Overview

Uniprot IDP07099
Protein NameEpoxide hydrolase 1
Gene NameEPHX1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
104 WRNEFDWKKQVEILN
105 RNEFDWKKQVEILNR
117 LNRYPHFKTKIEGLD
119 RYPHFKTKIEGLDIH
131 DIHFIHVKPPQLPAG
196 GFSEASSKKGFNSVA
197 FSEASSKKGFNSVAT
210 ATARIFYKLMLRLGF
286 VELLYPVKEKVFYSL
288 LLYPVKEKVFYSLMR
375 ISSQRFYKENLGQGW
386 GQGWMTQKHERMKVY
447 LLAQDIRKFLSVLER
52 DDSIRPFKVETSDEE
91 GFNSNYLKKVISYWR
92 FNSNYLKKVISYWRN

Function

Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water (By similarity). Plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids (PubMed:22798687). Metabolizes the abundant endocannabinoid 2-arachidonoylglycerol (2-AG) to free arachidonic acid (AA) and glycerol (PubMed:24958911). Binds 20(S)-hydroxycholesterol (20(S)-OHC) (By similarity)

Protein Sequence

10 MWLEILLTSV 20 LGFAIYWFIS 30 RDKEETLPLE 40 DGWWGPGTRS 50 AAREDDSIRP 60 FKVETSDEEI 70 HDLHQRIDKF 80 RFTPPLEDSC 90 FHYGFNSNYL 100 KKVISYWRNE 110 FDWKKQVEIL 120 NRYPHFKTKI 130 EGLDIHFIHV 140 KPPQLPAGHT 150 PKPLLMVHGW 160 PGSFYEFYKI 170 IPLLTDPKNH 180 GLSDEHVFEV 190 ICPSIPGYGF 200 SEASSKKGFN 210 SVATARIFYK 220 LMLRLGFQEF 230 YIQGGDWGSL 240 ICTNMAQLVP 250 SHVKGLHLNM 260 ALVLSNFSTL 270 TLLLGQRFGR 280 FLGLTERDVE 290 LLYPVKEKVF 300 YSLMRESGYM 310 HIQCTKPDTV 320 GSALNDSPVG 330 LAAYILEKFS 340 TWTNTEFRYL 350 EDGGLERKFS 360 LDDLLTNVML 370 YWTTGTIISS 380 QRFYKENLGQ 390 GWMTQKHERM 400 KVYVPTGFSA 410 FPFELLHTPE 420 KWVRFKYPKL 430 ISYSYMVRGG 440 HFAAFEEPEL 450 LAQDIRKFLS VLERQ

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005789 endoplasmic reticulum membrane
Molecular Function GO:0033961 cis-stilbene-oxide hydrolase activity
Molecular Function GO:0004301 epoxide hydrolase activity
Molecular Function GO:0008142 oxysterol binding
Biological Process GO:0019369 arachidonate metabolic process
Biological Process GO:0097176 epoxide metabolic process
Biological Process GO:0009636 response to toxic substance
Biological Process GO:0006805 xenobiotic metabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[4] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.