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Overview

Uniprot IDP07205
Protein NamePhosphoglycerate kinase 2
Gene NamePGK2
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
11 SKKLTLDKLDVRGKR
141 DPSGKKIKAEPDKIE
192 ASGFLMKKELDYFAK
220 GGAKVADKIQLIKNM
267 EEGAKIVKDIMAKAQ
272 IVKDIMAKAQKNGVR
31 DFNVPMKKNQITNNQ
322 DCGPESNKNHAQVVA
353 DAFAKGTKALMDEIV
41 ITNNQRIKASIPSIK
6 **MSLSKKLTLDKLD
91 ELKSLLGKDVLFLKD

Function

Catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate and plays a role in glycolysis and gluconeogenesis (By similarity). Essential for sperm motility and male fertility (PubMed:26677959). Not required for the completion of spermatogenesis (By similarity)

Protein Sequence

10 MSLSKKLTLD 20 KLDVRGKRVI 30 MRVDFNVPMK 40 KNQITNNQRI 50 KASIPSIKYC 60 LDNGAKAVVL 70 MSHLGRPDGV 80 PMPDKYSLAP 90 VAVELKSLLG 100 KDVLFLKDCV 110 GAEVEKACAN 120 PAPGSVILLE 130 NLRFHVEEEG 140 KGQDPSGKKI 150 KAEPDKIEAF 160 RASLSKLGDV 170 YVNDAFGTAH 180 RAHSSMVGVN 190 LPHKASGFLM 200 KKELDYFAKA 210 LENPVRPFLA 220 ILGGAKVADK 230 IQLIKNMLDK 240 VNEMIIGGGM 250 AYTFLKVLNN 260 MEIGASLFDE 270 EGAKIVKDIM 280 AKAQKNGVRI 290 TFPVDFVTGD 300 KFDENAQVGK 310 ATVASGISPG 320 WMGLDCGPES 330 NKNHAQVVAQ 340 ARLIVWNGPL 350 GVFEWDAFAK 360 GTKALMDEIV 370 KATSKGCITV 380 IGGGDTATCC 390 AKWNTEDKVS 400 HVSTGGGASL 410 ELLEGKILPG VEALSNM

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005634 nucleus
Cellular Component GO:0035686 sperm fibrous sheath
Molecular Function GO:0043531 ADP binding
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0004618 phosphoglycerate kinase activity
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0006094 gluconeogenesis
Biological Process GO:0006096 glycolytic process
Biological Process GO:0016310 phosphorylation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Yan M, Tu H, Tang S, Gai Z, Shi Q et al.. Lactylated Proteomic Analysis Reveals Functional Implications of Lysine Lactylation In Asthenozoospermia.. Mol Cell Proteomics 24(12):101439. 2025 Dec. PMID: 41192556.