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Overview

Uniprot IDP07335
Protein NameCreatine kinase B-type
Gene NameCkb
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
11 SNSHNTQKLRFPAED
242 LRVISMQKGGNMKEV
247 MQKGGNMKEVFTRFC
298 LRAGVHIKLPHLGKH
304 IKLPHLGKHEKFSEV
307 PHLGKHEKFSEVLKR
313 EKFSEVLKRLRLQKR

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work

Protein Sequence

10 MPFSNSHNTQ 20 KLRFPAEDEF 30 PDLSSHNNHM 40 AKVLTPELYA 50 ELRAKCTPSG 60 FTLDDAIQTG 70 VDNPGHPYIM 80 TVGAVAGDEE 90 SYDVFKDLFD 100 PIIEDRHGGY 110 QPSDEHKTDL 120 NPDNLQGGDD 130 LDPNYVLSSR 140 VRTGRSIRGF 150 CLPPHCSRGE 160 RRAIEKLAVE 170 ALSSLDGDLS 180 GRYYALKSMT 190 EAEQQQLIDD 200 HFLFDKPVSP 210 LLLASGMARD 220 WPDARGIWHN 230 DNKTFLVWIN 240 EEDHLRVISM 250 QKGGNMKEVF 260 TRFCTGLTQI 270 ETLFKSKNYE 280 FMWNPHLGYI 290 LTCPSNLGTG 300 LRAGVHIKLP 310 HLGKHEKFSE 320 VLKRLRLQKR 330 GTGGVDTAAV 340 GGVFDVSNAD 350 RLGFSEVELV 360 QMVVDGVKLL 370 IEMEQRLEQG 380 QPIDDLMPAQ K

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0030425 dendrite
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0043025 neuronal cell body
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004111 creatine kinase activity
Molecular Function GO:0031625 ubiquitin protein ligase binding
Biological Process GO:0071391 cellular response to estrogen stimulus
Biological Process GO:1904568 cellular response to wortmannin
Biological Process GO:0021549 cerebellum development
Biological Process GO:0140651 futile creatine cycle
Biological Process GO:0030644 intracellular chloride ion homeostasis
Biological Process GO:0046314 phosphocreatine biosynthetic process

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[3] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.