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Overview

Uniprot IDP07602
Protein NameProsaposin
Gene NamePSAP
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
414 GFCEVCKKLVGYLDR

Function

Saposins are specific low-molecular mass non-enzymatic glycoproteins that act as activator proteins for lysosomal sphingolipid-degrading enzymes, facilitating the hydrolysis of sphingolipids by extracting lipid substrates from membranes and presenting them to their respective enzymes. They are derived from a common precursor protein, prosaposin, which is proteolytically cleaved to yield four homologous proteins (saposin A, B, C, and D), each with distinct but partially overlapping lipid and enzyme specificities

Protein Sequence

10 MYALFLLASL 20 LGAALAGPVL 30 GLKECTRGSA 40 VWCQNVKTAS 50 DCGAVKHCLQ 60 TVWNKPTVKS 70 LPCDICKDVV 80 TAAGDMLKDN 90 ATEEEILVYL 100 EKTCDWLPKP 110 NMSASCKEIV 120 DSYLPVILDI 130 IKGEMSRPGE 140 VCSALNLCES 150 LQKHLAELNH 160 QKQLESNKIP 170 ELDMTEVVAP 180 FMANIPLLLY 190 PQDGPRSKPQ 200 PKDNGDVCQD 210 CIQMVTDIQT 220 AVRTNSTFVQ 230 ALVEHVKEEC 240 DRLGPGMADI 250 CKNYISQYSE 260 IAIQMMMHMQ 270 PKEICALVGF 280 CDEVKEMPMQ 290 TLVPAKVASK 300 NVIPALELVE 310 PIKKHEVPAK 320 SDVYCEVCEF 330 LVKEVTKLID 340 NNKTEKEILD 350 AFDKMCSKLP 360 KSLSEECQEV 370 VDTYGSSILS 380 ILLEEVSPEL 390 VCSMLHLCSG 400 TRLPALTVHV 410 TQPKDGGFCE 420 VCKKLVGYLD 430 RNLEKNSTKQ 440 EILAALEKGC 450 SFLPDPYQKQ 460 CDQFVAEYEP 470 VLIEILVEVM 480 DPSFVCLKIG 490 ACPSAHKPLL 500 GTEKCIWGPS 510 YWCQNTETAA 520 QCNAVEHCKR HVWN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0035577 azurophil granule membrane
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005576 extracellular region
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0005770 late endosome
Cellular Component GO:0043202 lysosomal lumen
Cellular Component GO:0005765 lysosomal membrane
Cellular Component GO:0005764 lysosome
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0008047 enzyme activator activity
Molecular Function GO:1905573 ganglioside GM1 binding
Molecular Function GO:1905574 ganglioside GM2 binding
Molecular Function GO:1905575 ganglioside GM3 binding
Molecular Function GO:1905577 ganglioside GP1c binding
Molecular Function GO:1905576 ganglioside GT1b binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0005543 phospholipid binding
Molecular Function GO:0002020 protease binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0097110 scaffold protein binding
Biological Process GO:0007193 adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
Biological Process GO:0060742 epithelial cell differentiation involved in prostate gland development
Biological Process GO:1905572 ganglioside GM1 transport to membrane
Biological Process GO:0010467 gene expression
Biological Process GO:0007041 lysosomal transport
Biological Process GO:0060736 prostate gland growth
Biological Process GO:0010506 regulation of autophagy
Biological Process GO:0019216 regulation of lipid metabolic process
Biological Process GO:0006665 sphingolipid metabolic process

Reference

[1] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.