Search Results

Overview

Uniprot IDP07686
Protein NameBeta-hexosaminidase subunit beta
Gene NameHEXB
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
161 KEPVAVLKANRVWGA
217 SRHYLPVKIILKTLD
382 GFGTDFKKLESFYIQ
412 WQEVFDDKAKLAPGT
465 QDWRKYYKVEPLDFG
476 LDFGGTQKQKQLFIG

Function

Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides (PubMed:11707436, PubMed:8123671, PubMed:8672428, PubMed:9694901). The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide (PubMed:11707436). Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A (PubMed:8123671, PubMed:8672428, PubMed:9694901). During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida (By similarity)

Protein Sequence

10 MELCGLGLPR 20 PPMLLALLLA 30 TLLAAMLALL 40 TQVALVVQVA 50 EAARAPSVSA 60 KPGPALWPLP 70 LLVKMTPNLL 80 HLAPENFYIS 90 HSPNSTAGPS 100 CTLLEEAFRR 110 YHGYIFGFYK 120 WHHEPAEFQA 130 KTQVQQLLVS 140 ITLQSECDAF 150 PNISSDESYT 160 LLVKEPVAVL 170 KANRVWGALR 180 GLETFSQLVY 190 QDSYGTFTIN 200 ESTIIDSPRF 210 SHRGILIDTS 220 RHYLPVKIIL 230 KTLDAMAFNK 240 FNVLHWHIVD 250 DQSFPYQSIT 260 FPELSNKGSY 270 SLSHVYTPND 280 VRMVIEYARL 290 RGIRVLPEFD 300 TPGHTLSWGK 310 GQKDLLTPCY 320 SRQNKLDSFG 330 PINPTLNTTY 340 SFLTTFFKEI 350 SEVFPDQFIH 360 LGGDEVEFKC 370 WESNPKIQDF 380 MRQKGFGTDF 390 KKLESFYIQK 400 VLDIIATINK 410 GSIVWQEVFD 420 DKAKLAPGTI 430 VEVWKDSAYP 440 EELSRVTASG 450 FPVILSAPWY 460 LDLISYGQDW 470 RKYYKVEPLD 480 FGGTQKQKQL 490 FIGGEACLWG 500 EYVDATNLTP 510 RLWPRASAVG 520 ERLWSSKDVR 530 DMDDAYDRLT 540 RHRCRMVERG 550 IAAQPLYAGY CNHENM

Gene Ontology

Classification GO ID Description
Cellular Component GO:0001669 acrosomal vesicle
Cellular Component GO:0042582 azurophil granule
Cellular Component GO:0035578 azurophil granule lumen
Cellular Component GO:1905379 beta-N-acetylhexosaminidase complex
Cellular Component GO:0060473 cortical granule
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005576 extracellular region
Cellular Component GO:0043202 lysosomal lumen
Cellular Component GO:0005764 lysosome
Cellular Component GO:0016020 membrane
Molecular Function GO:0008375 acetylglucosaminyltransferase activity
Molecular Function GO:0016231 beta-N-acetylglucosaminidase activity
Molecular Function GO:0004563 beta-N-acetylhexosaminidase activity
Molecular Function GO:0030246 carbohydrate binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0044877 protein-containing complex binding
Biological Process GO:0043615 astrocyte cell migration
Biological Process GO:0005975 carbohydrate metabolic process
Biological Process GO:0030207 chondroitin sulfate proteoglycan catabolic process
Biological Process GO:0030209 dermatan sulfate proteoglycan catabolic process
Biological Process GO:0006689 ganglioside catabolic process
Biological Process GO:0030203 glycosaminoglycan metabolic process
Biological Process GO:0030214 hyaluronan catabolic process
Biological Process GO:0006044 N-acetylglucosamine metabolic process
Biological Process GO:0006491 N-glycan processing
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0008360 regulation of cell shape
Biological Process GO:0007338 single fertilization

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.