Search Results

Overview

Uniprot IDP08237
Protein NameATP-dependent 6-phosphofructokinase, muscle type
Gene NamePFKM
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
615 NVEHLVQKMKTTVKR
678 FDRNFATKMGAKAMN
690 AMNWMSGKIKESYRN

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Protein Sequence

10 MTHEEHHAAK 20 TLGIGKAIAV 30 LTSGGDAQGM 40 NAAVRAVVRV 50 GIFTGARVFF 60 VHEGYQGLVD 70 GGDHIKEATW 80 ESVSMMLQLG 90 GTVIGSARCK 100 DFREREGRLR 110 AAYNLVKRGI 120 TNLCVIGGDG 130 SLTGADTFRS 140 EWSDLLSDLQ 150 KAGKITDEEA 160 TKSSYLNIVG 170 LVGSIDNDFC 180 GTDMTIGTDS 190 ALHRIMEIVD 200 AITTTAQSHQ 210 RTFVLEVMGR 220 HCGYLALVTS 230 LSCGADWVFI 240 PECPPDDDWE 250 EHLCRRLSET 260 RTRGSRLNII 270 IVAEGAIDKN 280 GKPITSEDIK 290 NLVVKRLGYD 300 TRVTVLGHVQ 310 RGGTPSAFDR 320 ILGSRMGVEA 330 VMALLEGTPD 340 TPACVVSLSG 350 NQAVRLPLME 360 CVQVTKDVTK 370 AMDEKKFDEA 380 LKLRGRSFMN 390 NWEVYKLLAH 400 VRPPVSKSGS 410 HTVAVMNVGA 420 PAAGMNAAVR 430 STVRIGLIQG 440 NRVLVVHDGF 450 EGLAKGQIEE 460 AGWSYVGGWT 470 GQGGSKLGTK 480 RTLPKKSFEQ 490 ISANITKFNI 500 QGLVIIGGFE 510 AYTGGLELME 520 GRKQFDELCI 530 PFVVIPATVS 540 NNVPGSDFSV 550 GADTALNTIC 560 TTCDRIKQSA 570 AGTKRRVFII 580 ETMGGYCGYL 590 ATMAGLAAGA 600 DAAYIFEEPF 610 TIRDLQANVE 620 HLVQKMKTTV 630 KRGLVLRNEK 640 CNENYTTDFI 650 FNLYSEEGKG 660 IFDSRKNVLG 670 HMQQGGSPTP 680 FDRNFATKMG 690 AKAMNWMSGK 700 IKESYRNGRI 710 FANTPDSGCV 720 LGMRKRALVF 730 QPVAELKDQT 740 DFEHRIPKEQ 750 WWLKLRPILK 760 ILAKYEIDLD 770 TSDHAHLEHI 780 TRKRSGEAAV

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005945 6-phosphofructokinase complex
Cellular Component GO:0016324 apical plasma membrane
Cellular Component GO:0005829 cytosol
Cellular Component GO:0016020 membrane
Cellular Component GO:0005634 nucleus
Cellular Component GO:0097228 sperm principal piece
Molecular Function GO:0003872 6-phosphofructokinase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0070061 fructose binding
Molecular Function GO:0070095 fructose-6-phosphate binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0019900 kinase binding
Molecular Function GO:0046872 metal ion binding
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0030388 fructose 1,6-bisphosphate metabolic process
Biological Process GO:0006002 fructose 6-phosphate metabolic process
Biological Process GO:0006096 glycolytic process
Biological Process GO:0061615 glycolytic process through fructose-6-phosphate
Biological Process GO:0046716 muscle cell cellular homeostasis
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[5] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[6] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[7] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[8] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[9] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[10] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[11] Yan M, Tu H, Tang S, Gai Z, Shi Q et al.. Lactylated Proteomic Analysis Reveals Functional Implications of Lysine Lactylation In Asthenozoospermia.. Mol Cell Proteomics 24(12):101439. 2025 Dec. PMID: 41192556.

[12] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.