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Overview

Uniprot ID	P08461
Protein Name	Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Gene Name	Dlat
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
348	VFVSPLAKKLAAEKG
354	AKKLAAEKGIDLTQV
362	GIDLTQVKGTGPEGR
373	PEGRIIKKDIDSFVP
451	LVRKELNKMLEGKGK
458	KMLEGKGKISVNDFI

Function

The pyruvate dehydrogenase (PDH) complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3);. Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acetyl groups from acetyl-lipoyl moiety generated by the pyruvate dehydrogenase, leading to acetyl-CoA formation

Protein Sequence

10 MWRVCARRVQ 20 SAVPRAGFRA 30 RWATLKGPRT 40 GPAAVRCGSG 50 IPSYGVRSLC 60 GWSYGSATVP 70 RNRILQQLLG 80 SPSRRSYSLP 90 PHQKVPLPSL 100 SPTMQAGTIA 110 RWEKKEGEKI 120 SEGDLIAEVE 130 TDKATVGFES 140 LEECYMAKIL 150 VPEGTRDVPV 160 GSIICITVEK 170 PQDIEAFKNY 180 TLDSATAATQ 190 AAPAPAAAPA 200 AAPAAPSASA 210 PGSSYPVHMQ 220 IVLPALSPTM 230 TMGTVQRWEK 240 KVGEKLSEGD 250 LLAEIETDKA 260 TIGFEVQEEG 270 YLAKILVPEG 280 TRDVPLGTPL 290 CIIVEKQEDI 300 AAFADYRPTE 310 VTSLKPQAPP 320 PVPPPVAAVP 330 PIPQPLAPTP 340 SAAPAGPKGR 350 VFVSPLAKKL 360 AAEKGIDLTQ 370 VKGTGPEGRI 380 IKKDIDSFVP 390 TKAAPAAAAA 400 APPGPRVAPT 410 PAGVFIDIPI 420 SNIRRVIAQR 430 LMQSKQTIPH 440 YYLSVDVNMG 450 EVLLVRKELN 460 KMLEGKGKIS 470 VNDFIIKASA 480 LACLKVPEAN 490 SSWMDTVIRQ 500 NHVVDVSVAV 510 STPAGLITPI 520 VFNAHIKGLE 530 TIASDVVSLA 540 SKAREGKLQP 550 HEFQGGTFTI 560 SNLGMFGIKN 570 FSAIINPPQA 580 CILAIGASED 590 KLIPADNEKG 600 FDVASVMSVT 610 LSCDHRVVDG 620 AVGAQWLAEF 630 KKYLEKPVTM LL

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005759	mitochondrial matrix
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0045254	pyruvate dehydrogenase complex
Molecular Function	GO:0016407	acetyltransferase activity
Molecular Function	GO:0004742	dihydrolipoyllysine-residue acetyltransferase activity
Molecular Function	GO:0042802	identical protein binding
Molecular Function	GO:0000166	nucleotide binding
Biological Process	GO:0006006	glucose metabolic process
Biological Process	GO:0042867	pyruvate catabolic process
Biological Process	GO:0006086	pyruvate decarboxylation to acetyl-CoA
Biological Process	GO:0006099	tricarboxylic acid cycle

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.