Overview
| Uniprot ID | P08754 |
|---|
| Protein Name | Guanine nucleotide-binding protein G(i) subunit alpha-3 |
|---|
| Gene Name | GNAI3 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 67 |
GYSEDECKQYKVVVY |
| 92 |
IRAMGRLKIDFGEAA |
Function
Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (By similarity). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:18434541, PubMed:19478087, PubMed:8774883). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K(+) channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935). The active GTP-bound form activates the calcium permeant TRPC5 ion channels (PubMed:37137991)
Protein Sequence
10
MGCTLSAEDK
20
AAVERSKMID
30
RNLREDGEKA
40
AKEVKLLLLG
50
AGESGKSTIV
60
KQMKIIHEDG
70
YSEDECKQYK
80
VVVYSNTIQS
90
IIAIIRAMGR
100
LKIDFGEAAR
110
ADDARQLFVL
120
AGSAEEGVMT
130
PELAGVIKRL
140
WRDGGVQACF
150
SRSREYQLND
160
SASYYLNDLD
170
RISQSNYIPT
180
QQDVLRTRVK
190
TTGIVETHFT
200
FKDLYFKMFD
210
VGGQRSERKK
220
WIHCFEGVTA
230
IIFCVALSDY
240
DLVLAEDEEM
250
NRMHESMKLF
260
DSICNNKWFT
270
ETSIILFLNK
280
KDLFEEKIKR
290
SPLTICYPEY
300
TGSNTYEEAA
310
AYIQCQFEDL
320
NRRKDTKEIY
330
THFTCATDTK
340
NVQFVFDAVT
350
DVIIKNNLKE
CGLY
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005813 |
centrosome |
| Cellular Component |
GO:0036064 |
ciliary basal body |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0005794 |
Golgi apparatus |
| Cellular Component |
GO:0005834 |
heterotrimeric G-protein complex |
| Cellular Component |
GO:0005765 |
lysosomal membrane |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0030496 |
midbody |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0001664 |
G protein-coupled receptor binding |
| Molecular Function |
GO:0031683 |
G-protein beta/gamma-subunit complex binding |
| Molecular Function |
GO:0019003 |
GDP binding |
| Molecular Function |
GO:0005525 |
GTP binding |
| Molecular Function |
GO:0003924 |
GTPase activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Biological Process |
GO:0007193 |
adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| Biological Process |
GO:0007188 |
adenylate cyclase-modulating G protein-coupled receptor signaling pathway |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0046039 |
GTP metabolic process |
| Biological Process |
GO:0007194 |
negative regulation of adenylate cyclase activity |
| Biological Process |
GO:0016239 |
positive regulation of macroautophagy |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.