Overview
| Uniprot ID | P09471 |
|---|
| Protein Name | Guanine nucleotide-binding protein G(o) subunit alpha |
|---|
| Gene Name | GNAO1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 105 |
KERKADAKMVCDVVS |
| 154 |
YQLNDSAKYYLDSLD |
| 181 |
DILRTRVKTTGIVET |
| 193 |
VETHFTFKNLHFRLF |
| 24 |
KAIEKNLKEDGISAA |
| 272 |
IILFLNKKDLFGEKI |
| 32 |
EDGISAAKDVKLLLL |
| 35 |
ISAAKDVKLLLLGAG |
| 46 |
LGAGESGKSTIVKQM |
| 51 |
SGKSTIVKQMKIIHE |
| 54 |
STIVKQMKIIHEDGF |
| 67 |
GFSGEDVKQYKPVVY |
| 98 |
LGIEYGDKERKADAK |
Function
Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed:29925951, PubMed:33408414, PubMed:39103320). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed:39103320). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed:39103320). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (By similarity). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (By similarity). Signaling is mediated via effector proteins, such as adenylate cyclase (By similarity). Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (By similarity)
Protein Sequence
10
MGCTLSAEER
20
AALERSKAIE
30
KNLKEDGISA
40
AKDVKLLLLG
50
AGESGKSTIV
60
KQMKIIHEDG
70
FSGEDVKQYK
80
PVVYSNTIQS
90
LAAIVRAMDT
100
LGIEYGDKER
110
KADAKMVCDV
120
VSRMEDTEPF
130
SAELLSAMMR
140
LWGDSGIQEC
150
FNRSREYQLN
160
DSAKYYLDSL
170
DRIGAADYQP
180
TEQDILRTRV
190
KTTGIVETHF
200
TFKNLHFRLF
210
DVGGQRSERK
220
KWIHCFEDVT
230
AIIFCVALSG
240
YDQVLHEDET
250
TNRMHESLML
260
FDSICNNKFF
270
IDTSIILFLN
280
KKDLFGEKIK
290
KSPLTICFPE
300
YTGPNTYEDA
310
AAYIQAQFES
320
KNRSPNKEIY
330
CHMTCATDTN
340
NIQVVFDAVT
350
DIIIANNLRG
CGLY
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0044297 |
cell body |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0030425 |
dendrite |
| Cellular Component |
GO:0098982 |
GABA-ergic synapse |
| Cellular Component |
GO:0098978 |
glutamatergic synapse |
| Cellular Component |
GO:0005834 |
heterotrimeric G-protein complex |
| Cellular Component |
GO:0098688 |
parallel fiber to Purkinje cell synapse |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0045211 |
postsynaptic membrane |
| Cellular Component |
GO:0042734 |
presynaptic membrane |
| Molecular Function |
GO:0051430 |
corticotropin-releasing hormone receptor 1 binding |
| Molecular Function |
GO:0003925 |
G protein activity |
| Molecular Function |
GO:0031821 |
G protein-coupled serotonin receptor binding |
| Molecular Function |
GO:0031683 |
G-protein beta/gamma-subunit complex binding |
| Molecular Function |
GO:0005525 |
GTP binding |
| Molecular Function |
GO:0003924 |
GTPase activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0031852 |
mu-type opioid receptor binding |
| Biological Process |
GO:0007193 |
adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| Biological Process |
GO:0007198 |
adenylate cyclase-inhibiting serotonin receptor signaling pathway |
| Biological Process |
GO:0007188 |
adenylate cyclase-modulating G protein-coupled receptor signaling pathway |
| Biological Process |
GO:0007212 |
G protein-coupled dopamine receptor signaling pathway |
| Biological Process |
GO:0006936 |
muscle contraction |
| Biological Process |
GO:0046676 |
negative regulation of insulin secretion |
| Biological Process |
GO:0099170 |
postsynaptic modulation of chemical synaptic transmission |
Reference
[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.