Search Results
Overview
| Uniprot ID | P09895 |
|---|---|
| Protein Name | Large ribosomal subunit protein uL18 |
| Gene Name | Rpl5 |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 178 | LSIPHSTKRFPGYDS |
| 197 | FNAEVHRKHIMGQNV |
| 220 | EEDEDAYKKQFSQYI |
| 221 | EDEDAYKKQFSQYIK |
| 255 | RENPVYEKKPKREVK |
| 27 | FRRRREGKTDYYARK |
| 270 | KKRWNRPKMSLAQKK |
| 276 | PKMSLAQKKDRVAQK |
| 283 | KKDRVAQKKASFLRA |
| 284 | KDRVAQKKASFLRAQ |
| 41 | KRLVIQDKNKYNTPK |
| 48 | KNKYNTPKYRMIVRV |
Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Molecular Function | GO:0008097 | 5S rRNA binding |
| Cellular Component | GO:0017101 | aminoacyl-tRNA synthetase multienzyme complex |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0022625 | cytosolic large ribosomal subunit |
| Cellular Component | GO:0022626 | cytosolic ribosome |
| Cellular Component | GO:0005783 | endoplasmic reticulum |
| Cellular Component | GO:0005730 | nucleolus |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0014069 | postsynaptic density |
| Cellular Component | GO:0032991 | protein-containing complex |
| Cellular Component | GO:1990904 | ribonucleoprotein complex |
| Cellular Component | GO:0045202 | synapse |
| Molecular Function | GO:0003730 | mRNA 3'-UTR binding |
| Molecular Function | GO:0048027 | mRNA 5'-UTR binding |
| Molecular Function | GO:0003729 | mRNA binding |
| Molecular Function | GO:0003735 | structural constituent of ribosome |
| Molecular Function | GO:1990948 | ubiquitin ligase inhibitor activity |
| Molecular Function | GO:0031625 | ubiquitin protein ligase binding |
| Biological Process | GO:0002181 | cytoplasmic translation |
| Biological Process | GO:2000059 | negative regulation of ubiquitin-dependent protein catabolic process |
| Biological Process | GO:0010922 | positive regulation of phosphatase activity |
| Biological Process | GO:0045727 | positive regulation of translation |
| Biological Process | GO:1901796 | regulation of signal transduction by p53 class mediator |
| Biological Process | GO:0000027 | ribosomal large subunit assembly |
| Biological Process | GO:0042273 | ribosomal large subunit biogenesis |
| Biological Process | GO:0006364 | rRNA processing |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.
[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.