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Overview

Uniprot IDP0C1X8
Protein NameAP2-associated protein kinase 1
Gene NameAak1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
169 VARLHQCKTPIIHRD
370 IAPRQRPKAGQTQPN
670 EASLSKSKSATTTPS

Function

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis (PubMed:11877461). Preferentially, may phosphorylate substrates on threonine residues (By similarity). Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes (By similarity). Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes (By similarity). Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (By similarity)

Protein Sequence

10 MKKFFDSRRE 20 QGSSGLGSGS 30 SGGGGSSSGL 40 GSGYIGRVFG 50 IGRQQVTVDE 60 VLAEGGFALV 70 FLVRTSNGVK 80 CALKRMFVNN 90 EHDLQVCKRE 100 IQIMRDLSGH 110 KNIVGYIDSS 120 INNVSSGDVW 130 EVLILMDFCR 140 GGQVVNLMNQ 150 RLQTGFTENE 160 VLQIFCDTCE 170 AVARLHQCKT 180 PIIHRDLKVE 190 NILLHDRGHY 200 VLCDFGSATN 210 KFQNPQAEGV 220 NAVEDEIKKY 230 TTLSYRAPEM 240 VNLYSGKIIT 250 TKADIWALGC 260 LLYKLCYFTL 270 PFGESQVAIC 280 DGSFTIPDNS 290 RYSQDMHCLI 300 YMLEPDPDKR 310 PDIYQVSYFS 320 FKLLKKECPV 330 PNVQNSPIPT 340 KLPEPVKASE 350 AAVKKTQPKA 360 RLTDPIPTTE 370 TSIAPRQRPK 380 AGQTQPNPGI 390 LPIQPALTPR 400 KRATVQPLPQ 410 ATGPSNQPSL 420 LASVSQPKAQ 430 ATPSQPLQSS 440 QPKQPQAPPT 450 PQQTPAPQTQ 460 GLPTQAQATP 470 QHQQQLLLKQ 480 QQQQQQQQQQ 490 QQPQQPTAPP 500 QPSGTFYQQQ 510 QPQQQQAQTQ 520 QQFQAVHPAA 530 QQSVTAQFPV 540 VSQGGSQQQL 550 MQNFYQQQQQ 560 QQQQQQQLMA 570 QQAALQQKTA 580 VVVPQPQAQP 590 ATAPQAAAAQ 600 EPQIQAPARQ 610 QPKVQTTPPP 620 TIQGQKVGSL 630 TPPSSPKTQR 640 AGHRRILSDV 650 THSAVFGVPA 660 SKSTQLLHAA 670 AAEASLSKSK 680 SATTTPSGSP 690 RTSQQNVSNA 700 SEGSTWNPFD 710 DDNFSKLTAE 720 ELLNKDFAKL 730 GEGKLPEKLG 740 GSAESLIPGF 750 QATQGDAFAT 760 SSFSAGTAEK 770 RKGGQAVDSG 780 IPLLSVSDPF 790 IPLQVPDAPE 800 KLIEGLKSPD 810 TSLLLPDLLP 820 MTDPFGSTSD 830 AVIEKADAAV 840 ESLIPGLEPP 850 VAQRLPSHTE 860 SVTSNRTDSL 870 TGEDSLLDCS 880 LLSNPTADLL 890 DEFAPIALSA 900 STHKAAEDSN 910 LISGFGVAEG 920 SEKVAEDEFD 930 PIPVLITKNT 940 QGGHSRNSSG 950 SSESSLPNLA 960 RSLLLVDQLI DL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0044305 calyx of Held
Cellular Component GO:0031252 cell leading edge
Cellular Component GO:0071439 clathrin complex
Cellular Component GO:0005905 clathrin-coated pit
Cellular Component GO:0030136 clathrin-coated vesicle
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0098793 presynapse
Cellular Component GO:0043195 terminal bouton
Molecular Function GO:0035612 AP-2 adaptor complex binding
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0005112 Notch binding
Molecular Function GO:0106310 protein serine kinase activity
Molecular Function GO:0004674 protein serine/threonine kinase activity
Biological Process GO:0045747 positive regulation of Notch signaling pathway
Biological Process GO:0140238 presynaptic endocytosis
Biological Process GO:0006468 protein phosphorylation
Biological Process GO:0050821 protein stabilization
Biological Process GO:2000369 regulation of clathrin-dependent endocytosis
Biological Process GO:0032880 regulation of protein localization

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.