Search Results

Overview

Uniprot ID	P10888
Protein Name	Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
Gene Name	Cox4i1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
143	DWVAMQTKRMLDMKV
29	RAHGSVVKSEDYALP
60	KLLSASQKALKEKEK
67	KALKEKEKADWSSLS
78	SSLSRDEKVQLYRIQ

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix

Protein Sequence

10 MLATRALSLI 20 GKRAISTSVC 30 LRAHGSVVKS 40 EDYALPSYVD 50 RRDYPLPDVA 60 HVKLLSASQK 70 ALKEKEKADW 80 SSLSRDEKVQ 90 LYRIQFNESF 100 AEMNKGTNEW 110 KTVVGLAMFF 120 IGFTALVLIW 130 EKSYVYGPIP 140 HTFDRDWVAM 150 QTKRMLDMKV 160 NPIQGFSAKW DYNKNEWKK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0031966	mitochondrial membrane
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0045277	respiratory chain complex IV
Biological Process	GO:0006123	mitochondrial electron transport, cytochrome c to oxygen
Biological Process	GO:0007584	response to nutrient

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[3] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.