Search Results

Overview

Uniprot IDP11182
Protein NameLipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Gene NameDBT
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
170 HQEIKGRKTLATPAV
196 SEVVGSGKDGRILKE
252 PVFTGKDKTEPIKGF

Function

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component

Protein Sequence

10 MAAVRMLRTW 20 SRNAGKLICV 30 RYFQTCGNVH 40 VLKPNYVCFF 50 GYPSFKYSHP 60 HHFLKTTAAL 70 RGQVVQFKLS 80 DIGEGIREVT 90 VKEWYVKEGD 100 TVSQFDSICE 110 VQSDKASVTI 120 TSRYDGVIKK 130 LYYNLDDIAY 140 VGKPLVDIET 150 EALKDSEEDV 160 VETPAVSHDE 170 HTHQEIKGRK 180 TLATPAVRRL 190 AMENNIKLSE 200 VVGSGKDGRI 210 LKEDILNYLE 220 KQTGAILPPS 230 PKVEIMPPPP 240 KPKDMTVPIL 250 VSKPPVFTGK 260 DKTEPIKGFQ 270 KAMVKTMSAA 280 LKIPHFGYCD 290 EIDLTELVKL 300 REELKPIAFA 310 RGIKLSFMPF 320 FLKAASLGLL 330 QFPILNASVD 340 ENCQNITYKA 350 SHNIGIAMDT 360 EQGLIVPNVK 370 NVQICSIFDI 380 ATELNRLQKL 390 GSVSQLSTTD 400 LTGGTFTLSN 410 IGSIGGTFAK 420 PVIMPPEVAI 430 GALGSIKAIP 440 RFNQKGEVYK 450 AQIMNVSWSA 460 DHRVIDGATM 470 SRFSNLWKSY 480 LENPAFMLLD LK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0160157 branched-chain alpha-ketoacid dehydrogenase complex
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0042645 mitochondrial nucleoid
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups
Molecular Function GO:0043754 dihydrolipoamide branched chain acyltransferase activity
Molecular Function GO:0031625 ubiquitin protein ligase binding
Biological Process GO:0120552 branched-chain alpha-keto acid decarboxylation to branched-chain acyl-CoA
Biological Process GO:0009083 branched-chain amino acid catabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.