Search Results

Overview

Uniprot IDP11310
Protein NameMedium-chain specific acyl-CoA dehydrogenase, mitochondrial
Gene NameACADM
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
212 ARSDPDPKAPANKAF
236 PGIQIGRKELNMGQR
259 FEDVKVPKENVLIGD
279 VAMGAFDKTRPVVAA
301 RALDEATKYALERKT
395 EKLMRDAKIYQIYEG

Function

Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175). The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed:2251268). ETF is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed:15159392, PubMed:25416781). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase has preference for fatty acyl-CoAs with saturated 6 to 12 carbons long primary chains, making it but can also catalyze longer chains such as C14 and C16 (PubMed:1970566, PubMed:21237683, PubMed:2251268, PubMed:8823175)

Protein Sequence

10 MAAGFGRCCR 20 VLRSISRFHW 30 RSQHTKANRQ 40 REPGLGFSFE 50 FTEQQKEFQA 60 TARKFAREEI 70 IPVAAEYDKT 80 GEYPVPLIRR 90 AWELGLMNTH 100 IPENCGGLGL 110 GTFDACLISE 120 ELAYGCTGVQ 130 TAIEGNSLGQ 140 MPIIIAGNDQ 150 QKKKYLGRMT 160 EEPLMCAYCV 170 TEPGAGSDVA 180 GIKTKAEKKG 190 DEYIINGQKM 200 WITNGGKANW 210 YFLLARSDPD 220 PKAPANKAFT 230 GFIVEADTPG 240 IQIGRKELNM 250 GQRCSDTRGI 260 VFEDVKVPKE 270 NVLIGDGAGF 280 KVAMGAFDKT 290 RPVVAAGAVG 300 LAQRALDEAT 310 KYALERKTFG 320 KLLVEHQAIS 330 FMLAEMAMKV 340 ELARMSYQRA 350 AWEVDSGRRN 360 TYYASIAKAF 370 AGDIANQLAT 380 DAVQILGGNG 390 FNTEYPVEKL 400 MRDAKIYQIY 410 EGTSQIQRLI 420 VAREHIDKYK N

Gene Ontology

Classification GO ID Description
Cellular Component GO:0030424 axon
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0031966 mitochondrial membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003995 acyl-CoA dehydrogenase activity
Molecular Function GO:0050660 flavin adenine dinucleotide binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0070991 medium-chain fatty acyl-CoA dehydrogenase activity
Biological Process GO:0045329 carnitine biosynthetic process
Biological Process GO:0019254 carnitine metabolic process, CoA-linked
Biological Process GO:0006635 fatty acid beta-oxidation
Biological Process GO:0033539 fatty acid beta-oxidation using acyl-CoA dehydrogenase
Biological Process GO:0005978 glycogen biosynthetic process
Biological Process GO:0051793 medium-chain fatty acid catabolic process
Biological Process GO:0051791 medium-chain fatty acid metabolic process
Biological Process GO:0006111 regulation of gluconeogenesis

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.